Rusyn5: Drug study: High-fat diet plus subchronic alcohol-induced effects in males of 14 inbred strains of mice (2011)

Tsuchiya M, Ji C, Kosyk O, Shymonyak S, Melnyk S, Kono H, Tryndyak V, Muskhelishvili L, Pogribny IP, Kaplowitz N, Rusyn I. Interstrain differences in liver injury and one-carbon metabolism in alcohol-fed mice. Hepatology. 2012 Jul;56(1):130-9. doi: 10.1002/hep.2564Epub 2012 Jun 6.   PubMed 22307928     FullText


       
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Participants Ivan Rusyn       Texas A&M University College Station, TX
With: Tsuchiya M, Ji C, Kosyk O, Shymonyak S, Melnyk S, Kono H, Tryndyak V, Muskhelishvili L, Pogribny IP, Kaplowitz N, Gatti DM, Kawaguchi A, Lam TC, Uehara T, Wright FA
ContactIvan Rusyn     IRusyn@cvm.tamu.edu     Lab web site
AcknowledgementsFunding provided by NIH AA016258, AA014428, AA018846, ES010126, ES015241
Project type Phenotype strain survey data set
MPD identifiersRusyn5     MPD:430
Data changelog 2 updates/corrections.       Initial release date: 07/2012.
Formatted citation
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The effects of high-fat diet on alcohol-induced liver injury were assessed using an intragastric intubation model. Urine alcohol content was monitored daily. Mice were evaluated for endoplasmic reticulum stress response as well as lipid and one-carbon metabolism. There were 2 cohorts: (1) mice fed high-fat diet only; (2) mice fed high-fat diet plus alcohol.

Summary of procedures that were conducted
• metabolic panel Alanine transaminase (serum) after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• alcohol quantification Alcohol concentration in blood and urine after 4 wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• organ weights Liver weight after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• histopathology Liver histopathology, injury after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• metabolite quantification Homocysteine concentration after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• lipid profile Hepatic triglycerides after 4wk regimen of ethanol and high-fat diet. 24h fast.
• RNA expression profiling Liver gene expression after 4wk regimen of ethanol and high-fat diet. 24h fast. mRNA abundance relative to the control (high-fat only) group.
Mice: inbred   14 strains   ♂   age 8-14wks   2 cohorts