MPD: Data set: Paigen3

Bouchard G, Johnson D, Carver T, Paigen B, Carey MC. Cholesterol gallstone formation in overweight mice establishes that obesity per se is not linked directly to cholelithiasis risk. J Lipid Res. 2002 Jul;43(7):1105-13. PubMed 12091495

All animals were fed a high-fat (15% fat) diet for 8 wks. Blood chemistry and cholesterol were measured. After sac, a pathology review of livers and gallbladders was conducted. A collection of liver images is available as part of this project.

Procedures conducted:

• body weight		Baseline vs. high-fat diet for 8wks.
• lipid profile		Cholesterol, HDL, non-HDL. 4h fast, plasma. Hepatic lipids. 8wks on high-fat diet.
• metabolic panel		Alanine transaminase, bile salts (plasma) after 8wks on high-fat diet. 4h fast.
• histopathology		Gallstone, gallbladder, and atherosclerosis lesion histopathology after 8wks on high-fat diet
• organ dimensions		Gallbladder volume after 8wks on high-fat diet
• organ weights		Liver weight after 8wks on high-fat diet.

Paigen3 downloads
• Download Paigen3 project data set animal data, as uploaded
• Download Paigen3 animal data matrix with factor-related expansion applied as necessary
• Download Paigen3 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Paigen3 measures:

MA:0000062 aorta
MA:0000356 gall bladder
MA:0000358 liver
MA:0002501 plasma
MA:0002513 bile
MP:0000180 abnormal circulating cholesterol level
MP:0000184 abnormal circulating HDL cholesterol level
MP:0000598 abnormal liver morphology
MP:0001259 abnormal body weight
MP:0001573 abnormal circulating alanine transaminase level
MP:0004773 abnormal bile composition
MP:0004774 abnormal bile salt level
MP:0004847 abnormal liver weight
MP:0005340 altered susceptibility to atherosclerosis
MP:0012776 abnormal liver cholesterol level
MP:0020098 abnormal susceptibility to diet-induced aortic fatty streak lesions
MP:0020131 abnormal gallbladder volume
MP:0020151 abnormal circulating non-HDL cholesterol level
VT:0000180 blood cholesterol amount
VT:0000184 blood HDL cholesterol amount
VT:0000272 aorta morphology trait
VT:0000598 liver morphology trait
VT:0000609 liver physiology trait
VT:0001259 body mass
VT:0001547 lipid amount
VT:0001573 blood alanine transaminase amount
VT:0003402 liver mass
VT:0005084 gall bladder morphology trait
VT:0005086 bile amount
VT:0005675 gall bladder size trait
VT:0010474 blood non-HDL cholesterol amount
VT:0015078 blood homeostasis trait

Investigators	Beverly Paigen The Jackson Laboratory, Bar Harbor, ME Guylaine Bouchard Hopital Ste-Justine, Montreal, Quebec CANADA Martin C Carey Harvard Medical School, Boston, MA
Participants	Johnson D, Tovbina M, Silva A, Carver T, Tracy T, McFarland C, Archer J, Whitmore H
Contact	Beverly Paigen bev.paigen@jax.org
Affiliated Center	JAX Heart, Lung, and Blood Center (HLB)
Part of a series:	• Inbred strains see Paigen1 • Obesity mutant strains (this project) Paigen3
Acknowledgements	Funding provided by NIH DK51568, HL66611, DK36588, DK34854, DK52911; Medical Research Council of Canada Generous funds from Aventis Pharmaceuticals Inc. were used to defray the cost of mice through the Mouse Phenome Project.
Project type	Phenotype strain survey data set
MPD identifiers	Paigen3 MPD:28
Data changelog	4 updates/corrections. Initial release date: 02/2001.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Paigen3:4	Paigen3	colony observation	(none)	age at start of diet (local parameter) [d] age 2804	obesity mutants	7	both		N=10

Paigen3:5	Paigen3	body weight	high-fat diet	body weight [g] bw 2805 start after 8wks on diet fold change	obesity mutants	8	both	7-11wks	N=25

Paigen3:7	Paigen3	organ weights	high-fat diet	liver weight at sacrifice [g] liverweight 2807	obesity mutants	8	both	15-19wks	N=20

Paigen3:8	Paigen3	organ dimensions	high-fat diet	gallbladder volume [µL] gbvol 2808	obesity mutants	8	both	15-19wks	N=18

Paigen3:9	Paigen3	histopathology	high-fat diet	gallbladder, mucin (score: 0-4) [score] mucin 2809	obesity mutants	7	both	15-19wks	N=8

Paigen3:10	Paigen3	histopathology	high-fat diet	gallbladder, arc (score: 0-4) [score] arc 2810	obesity mutants	7	both	15-19wks	N=8

Paigen3:11	Paigen3	histopathology	high-fat diet	gallbladder, filaments (score: 0-4) [score] filaments 2811	obesity mutants	7	both	15-19wks	N=8

Paigen3:12	Paigen3	histopathology	high-fat diet	gallbladder, small liquid crystals (score: 0-4) [score] smallLC 2812	obesity mutants	7	both	15-19wks	N=8

Paigen3:13	Paigen3	histopathology	high-fat diet	gallbladder, aggregated liquid crystals (score: 0-4) [score] agLC 2813	obesity mutants	7	both	15-19wks	N=8

Paigen3:14	Paigen3	histopathology	high-fat diet	gallbladder, fused liquid crystals (score: 0-4) [score] fusedLC 2814	obesity mutants	7	both	15-19wks	N=8

Paigen3:15	Paigen3	histopathology	high-fat diet	gallbladder, cholesterol monohydrate crystals (score: 0-4) [score] ChMC 2815	obesity mutants	7	both	15-19wks	N=8

Paigen3:16	Paigen3	histopathology	high-fat diet	gallbladder, number of sandy stones [n] sandysto 2816	obesity mutants	7	both	15-19wks	N=8

Paigen3:17	Paigen3	histopathology	high-fat diet	gallbladder, number of true gallstones [n] gs 2817	obesity mutants	7	both	15-19wks	N=8

Paigen3:18	Paigen3	histopathology	high-fat diet	gallbladder, number of sandy plus true stones [n] nstones 2818	obesity mutants	7	both	15-19wks	N=8

Paigen3:19	Paigen3	histopathology	high-fat diet	gallbladder, presence of stones (score: 1=present, 0=absent) [score] st_pres 2819	obesity mutants	8	both	15-19wks	N=19

Paigen3:20	Paigen3	lipid profile	high-fat diet	total cholesterol (plasma) [mg/dL] totalCh 2820	obesity mutants	8	both	15-19wks	N=17

Paigen3:21	Paigen3	lipid profile	high-fat diet	HDL cholesterol (plasma) [mg/dL] HDL_Ch 2821	obesity mutants	8	both	15-19wks	N=18

Paigen3:22	Paigen3	lipid profile	high-fat diet	plasma cholesterol not in the HDL fraction [mg/dL] nonHDL_Ch 2822	obesity mutants	8	both	15-19wks	N=16

Paigen3:23	Paigen3	lipid profile	high-fat diet	percentage of total plasma cholesterol in HDL fraction [%] pctHDL_TCH 2823	obesity mutants	8	both	15-19wks	N=16

Paigen3:24	Paigen3	metabolic panel	high-fat diet	alanine transaminase (plasma ALT) [IU/L] ALT 2824	obesity mutants	8	both	15-19wks	N=16

Paigen3:25	Paigen3	metabolic panel	high-fat diet	bile salts (plasma) [µmol/L] bilesalts 2825	obesity mutants	8	both	15-19wks	N=17

Paigen3:26	Paigen3	lipid profile	high-fat diet	hepatic cholesterol concentration (free per g liver) [mg] freeHCC 2826	obesity mutants	8	both	15-19wks	N=6

Paigen3:27	Paigen3	lipid profile	high-fat diet	hepatic cholesterol concentration (esterified per g liver) [mg] estHCC 2827	obesity mutants	8	both	15-19wks	N=6

Paigen3:28	Paigen3	lipid profile	high-fat diet	hepatic cholesterol concentration (total per g liver) [mg] totHCC 2828	obesity mutants	8	both	15-19wks	N=9

Paigen3:29	Paigen3	lipid profile	high-fat diet	hepatic cholesterol concentration (free to esterified ratio) [ratio] free_to_est 2829	obesity mutants	8	both	15-19wks	N=6

Paigen3:30	Paigen3	histopathology	high-fat diet	liver microvacuoles (score: 0-4) [score] microvacs 2830	obesity mutants	7	both	15-19wks	N=3

Paigen3:31	Paigen3	histopathology	high-fat diet	liver macrovacuoles (score: 0-4) [score] macrovacs 2831	obesity mutants	7	both	15-19wks	N=3

Paigen3:32	Paigen3	histopathology	high-fat diet	liver inflammation (score: 0-4) [score] inflammation 2832	obesity mutants	7	both	15-19wks	N=3

Paigen3:36	Paigen3	histopathology	high-fat diet	fatty streak aortic lesion size [µm²] aortic_lesion 2836	obesity mutants	7	both	15-19wks	N=8

Paigen3: Diet effects on gallstone formation, liver pathology, plasma lipids, and pathogen-accelerated atherosclerosis in 8 obesity mutant mouse strains (2000)

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