Paigen3: Diet effects on gallstone formation, liver pathology, plasma lipids, and pathogen-accelerated atherosclerosis in 8 obesity mutant mouse strains (2000)

Bouchard G, Johnson D, Carver T, Paigen B, Carey MC. Cholesterol gallstone formation in overweight mice establishes that obesity per se is not linked directly to cholelithiasis risk. J Lipid Res. 2002 Jul;43(7):1105-13.   PubMed 12091495  


       
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Participants Beverly Paigen       The Jackson Laboratory Bar Harbor, ME
Guylaine Bouchard       Hopital Ste-Justine Montreal, Quebec CANADA
Martin C Carey       Harvard Medical School Boston, MA
With: Johnson D, Tovbina M, Silva A, Carver T, Tracy T, McFarland C, Archer J, Whitmore H
ContactBeverly Paigen     bev.paigen@jax.org
AffiliationJAX Heart, Lung, and Blood Center (HLB)
Part of a series: • Inbred strains   see Paigen1
• Obesity mutant strains   (this project) Paigen3
AcknowledgementsFunding provided by NIH DK51568, HL66611, DK36588, DK34854, DK52911; Medical Research Council of Canada

Generous funds from Aventis Pharmaceuticals Inc. were used to defray the cost of mice through the Mouse Phenome Project.
Project type Phenotype strain survey data set
MPD identifiersPaigen3     MPD:28
Data changelog 4 updates/corrections.       Initial release date: 02/2001.
Formatted citation
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All animals were fed a high-fat (15% fat) diet for 8 wks. Blood chemistry and cholesterol were measured. After sac, a pathology review of livers and gallbladders was conducted. A collection of liver images is available as part of this project.

Summary of procedures that were conducted
• body weight Baseline vs. high-fat diet for 8wks.
• lipid profile Cholesterol, HDL, non-HDL. 4h fast, plasma. Hepatic lipids. 8wks on high-fat diet.
• metabolic panel Alanine transaminase, bile salts (plasma) after 8wks on high-fat diet. 4h fast.
• histopathology Gallstone, gallbladder, and atherosclerosis lesion histopathology after 8wks on high-fat diet
• organ dimensions Gallbladder volume after 8wks on high-fat diet
• organ weights Liver weight after 8wks on high-fat diet.
Mice: obesity mutants   8 strains   ♀♂   age 15-19wks   1 cohort

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Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Paigen3 2805 body weight high-fat diet body weight   [g]     start    after 8wks on diet    Δchange obesity mutants 8 both 7-11wks N=22
    Paigen3 2807 organ weights high-fat diet liver weight at sacrifice   [g]  liverweight obesity mutants 8 both 15-19wks N=20
    Paigen3 2808 organ dimensions high-fat diet gallbladder volume   [µL]  gbvol obesity mutants 8 both 15-19wks N=18
    Paigen3 2809 histopathology high-fat diet mucin (score: 0-4)   [score]  mucin obesity mutants 7 both 15-19wks N=8
    Paigen3 2810 histopathology high-fat diet arc (score: 0-4)   [score]  arc obesity mutants 7 both 15-19wks N=8
    Paigen3 2811 histopathology high-fat diet filaments (score: 0-4)   [score]  filaments obesity mutants 7 both 15-19wks N=8
    Paigen3 2812 histopathology high-fat diet small liquid crystals (score: 0-4)   [score]  smallLC obesity mutants 7 both 15-19wks N=8
    Paigen3 2813 histopathology high-fat diet aggregated liquid crystals (score: 0-4)   [score]  agLC obesity mutants 7 both 15-19wks N=8
    Paigen3 2814 histopathology high-fat diet fused liquid crystals (score: 0-4)   [score]  fusedLC obesity mutants 7 both 15-19wks N=8
    Paigen3 2815 histopathology high-fat diet cholesterol monohydrate crystals (score: 0-4)   [score]  ChMC obesity mutants 7 both 15-19wks N=8
    Paigen3 2816 histopathology high-fat diet number of sandy stones   [n]  sandysto obesity mutants 7 both 15-19wks N=8
    Paigen3 2817 histopathology high-fat diet number of true gallstones   [n]  gs obesity mutants 7 both 15-19wks N=8
    Paigen3 2818 histopathology high-fat diet number of sandy plus true stones   [n]  nstones obesity mutants 7 both 15-19wks N=8
    Paigen3 2819 histopathology high-fat diet presence of stones (score: 1=present, 0=absent)   [score]  st_pres obesity mutants 8 both 15-19wks N=19
    Paigen3 2820 lipid profile high-fat diet total cholesterol (plasma)   [mg/dL]  totalCh obesity mutants 8 both 15-19wks N=17
    Paigen3 2821 lipid profile high-fat diet HDL cholesterol (plasma)   [mg/dL]  HDL_Ch obesity mutants 8 both 15-19wks N=18
    Paigen3 2822 lipid profile high-fat diet plasma cholesterol not in the HDL fraction   [mg/dL]  nonHDL_Ch obesity mutants 8 both 15-19wks N=16
    Paigen3 2823 lipid profile high-fat diet percent of total plasma cholesterol in HDL fraction   [%]  pctHDL_TCH obesity mutants 8 both 15-19wks N=16
    Paigen3 2824 metabolic panel high-fat diet alanine transaminase (plasma ALT)   [IU/L]  ALT obesity mutants 8 both 15-19wks N=16
    Paigen3 2825 metabolic panel high-fat diet bile salts (plasma)   [µmol/L]  bilesalts obesity mutants 8 both 15-19wks N=17
    Paigen3 2826 lipid profile high-fat diet hepatic cholesterol concentration (free per g liver)   [mg]  freeHCC obesity mutants 8 both 15-19wks N=6
    Paigen3 2827 lipid profile high-fat diet hepatic cholesterol concentration (esterified per g liver)   [mg]  estHCC obesity mutants 8 both 15-19wks N=6
    Paigen3 2828 lipid profile high-fat diet hepatic cholesterol concentration (total per g liver)   [mg]  totHCC obesity mutants 8 both 15-19wks N=9
    Paigen3 2829 lipid profile high-fat diet hepatic cholesterol concentration (free to esterified ratio)   [ratio]  free_to_est obesity mutants 8 both 15-19wks N=6
    Paigen3 2830 histopathology high-fat diet microvacuoles (score: 0-4)   [score]  microvacs obesity mutants 7 both 15-19wks N=3
    Paigen3 2831 histopathology high-fat diet macrovacuoles (score: 0-4)   [score]  macrovacs obesity mutants 7 both 15-19wks N=3
    Paigen3 2832 histopathology high-fat diet inflammation (score: 0-4)   [score]  inflammation obesity mutants 7 both 15-19wks N=3
    Paigen3 2836 histopathology high-fat diet fatty streak aortic lesion size   [µm2]  aortic_lesion obesity mutants 7 both 15-19wks N=8
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