MPD: Data set: Chesler4

Chesler4: Behavioral and nociception traits in a Diversity Outbred (DO) mouse population and 8 founder inbred strains (2013)

Logan RW, Robledo RF, Recla JM, Philip VM, Bubier JA, Jay JJ, Harwood C, Wilcox T, Gatti DM, Bult CJ, Churchill GA, Chesler EJ. High-precision genetic mapping of behavioral traits in the diversity outbred mouse population. Genes Brain Behav. 2013 Jun;12(4):424-37. doi: 10.1111/gbb.12029. Epub 2013 Mar 20. PubMed 23433259

Recla JM, Robledo RF, Gatti DM, Bult CJ, Churchill GA, Chesler EJ. Precise genetic mapping and integrative bioinformatics in Diversity Outbred mice reveals Hydin as a novel pain gene. Mamm Genome. 2014 Jun;25(5-6):211-22. doi: 10.1007/s00335-014-9508-0. Epub 2014 Apr 5. PubMed 24700285 FullText

Procedures conducted:

• open field test		Locomotor activity, exploratory, anxiety-related behavior. 20 min test.
• visual cliff		Anxiety, avoidance behavior. 20 min test.
• light-dark box		Anxiety, avoidance behavior. 20 min test.
• tail suspension test		Frequency climbing, time immobile, latency immobile. 5 min test.
• nociception assay		Hot plate.
• body weight

Chesler4 downloads
• Download Chesler4 project data set animal data, as uploaded
• Download Chesler4 animal data matrix with factor-related expansion applied as necessary
• Download Chesler4 strain means, SD, N, etc. one row per strain/sex/measure
• Chesler4 supplementary data

Ontology terms mapped to Chesler4 measures:

MP:0001259 abnormal body weight
MP:0001362 abnormal anxiety-related response
MP:0001392 abnormal locomotor behavior
MP:0002733 abnormal thermal nociception
MP:0003107 abnormal response to novelty
MP:0003360 abnormal depression-related behavior
MP:0013189 abnormal exploration in a new environment
VT:0001259 body mass
VT:0001392 locomotor behavior trait
VT:0001970 pain threshold
VT:0002733 thermal nociception trait
VT:0010459 behavioral response to novel environment trait
VT:0010471 exploratory behavior trait
VT:0010716 anxiety-related behavior trait
VT:0015015 depression-related behavior trait

Investigators	Ryan W Logan The Jackson Laboratory, Bar Harbor, ME Raymond F Robledo The Jackson Laboratory, Bar Harbor, ME Jill M Recla The Jackson Laboratory, Bar Harbor, ME Elissa J Chesler The Jackson Laboratory, Bar Harbor, ME
Participants	Philip VM, Bubier JA, Jay JJ, Harwood C, Wilcox T, Gatti DM, Bult CJ, Churchill GA
Contact	Elissa J Chesler elissa.chesler@jax.org Lab web site
Acknowledgements	Funding provided by NIH GM076468, AG038070
Project type	Phenotype strain survey data set
MPD identifiers	Chesler4 MPD:470
Data changelog	No updates/corrections. Initial release date: 06/2013.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Mice: DO population w/par 9 strains ♀♂ age 12-16wks 1 experimental group

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Chesler4:1	Chesler4	open field test	(none)	distance traveled in first 4 min, response to novelty, 20 min test [cm] OF_distance_first4 47001	DO population w/par	9	both	12-16wks	N=8

Chesler4:2	Chesler4	open field test	(none)	total distance traveled, 20 min test [cm] OF_distance 47002	DO population w/par	9	both	12-16wks	N=8

Chesler4:3	Chesler4	open field test	(none)	percentage of time in corners, 20 min test [%] OF_corner_pct 47003	DO population w/par	9	both	12-16wks	N=8

Chesler4:4	Chesler4	open field test	(none)	percentage of time in periphery, 20 min test [%] OF_periphery_pct 47004	DO population w/par	9	both	12-16wks	N=8

Chesler4:5	Chesler4	open field test	(none)	percentage of time immobile, 20 min test [%] OF_immobile_pct 47005	DO population w/par	9	both	12-16wks	N=8

Chesler4:6	Chesler4	open field test	(none)	percentage of time in center, 20 min test [%] OF_center_pct 47006	DO population w/par	9	both	12-16wks	N=8

Chesler4:7	Chesler4	light-dark box	(none)	distance traveled in light compartment, 20 min test [cm] LD_distance_light 47007	DO population w/par	9	both	12-16wks	N=8

Chesler4:8	Chesler4	light-dark box	(none)	number of light-dark transitions, 20 min test [n] LD_transitions 47008	DO population w/par	9	both	12-16wks	N=8

Chesler4:9	Chesler4	light-dark box	(none)	time in light compartment first 4 min, response to novelty, 20 min test [s] LD_light_first4 47009	DO population w/par	9	both	12-16wks	N=8

Chesler4:10	Chesler4	light-dark box	(none)	percentage of time in light compartment, 20 min test [%] LD_light_pct 47010	DO population w/par	9	both	12-16wks	N=8

Chesler4:11	Chesler4	visual cliff	(none)	distance traveled in top during first 4 min, response to novelty, 20 min test [cm] VC_top_distance_first4 47011	DO population w/par	9	both	12-16wks	N=8

Chesler4:12	Chesler4	visual cliff	(none)	time in top during first 4 min, response to novelty, 20 min test [s] VC_top_time_first4 47012	DO population w/par	9	both	12-16wks	N=8

Chesler4:13	Chesler4	visual cliff	(none)	total distance traveled in top, 20 min test [cm] VC_top_distance 47013	DO population w/par	9	both	12-16wks	N=8

Chesler4:14	Chesler4	visual cliff	(none)	percentage of time in top, 20 min test [%] VC_top_time_pct 47014	DO population w/par	9	both	12-16wks	N=8

Chesler4:15	Chesler4	visual cliff	(none)	mean velocity in top, 20 min test [cm/s] VC_top_velocity 47015	DO population w/par	9	both	12-16wks	N=8

Chesler4:16	Chesler4	visual cliff	(none)	distance traveled in bottom during first 4 min (response to novelty), 20 min test [cm] VC_bottom_distance_first4 47016	DO population w/par	9	both	12-16wks	N=8

Chesler4:17	Chesler4	visual cliff	(none)	time in bottom during first 4 min, response to novelty, 20 min test [s] VC_bottom_time_first4 47017	DO population w/par	9	both	12-16wks	N=8

Chesler4:18	Chesler4	visual cliff	(none)	total distance traveled in bottom, 20 min test [cm] VC_bottom_distance 47018	DO population w/par	9	both	12-16wks	N=8

Chesler4:20	Chesler4	visual cliff	(none)	percentage of time in bottom, 20 min test [%] VC_bottom_time_pct 47020	DO population w/par	9	both	12-16wks	N=8

Chesler4:21	Chesler4	visual cliff	(none)	mean velocity in bottom, 20 min test [cm/s] VC_bottom_velocity 47021	DO population w/par	9	both	12-16wks	N=8

Chesler4:22	Chesler4	visual cliff	(none)	number of top to bottom transitions, 20 min test [n] VC_bottom_transitions 47022	DO population w/par	9	both	12-16wks	N=8

Chesler4:24	Chesler4	nociception assay	(none)	latency of hindpaw withdrawal, 55°C heat stimulation [s] HP_latency 47024	DO population w/par	9	both	12-16wks	N=8

Chesler4:26	Chesler4	tail suspension test	(none)	average frequency climbing, 5 min test [n] TS_frequency_climbing 47026	DO population w/par	9	both	12-16wks	N=8

Chesler4:27	Chesler4	tail suspension test	(none)	average time immobile, 5 min test [s] TS_time_immobile 47027	DO population w/par	9	both	12-16wks	N=8

Chesler4:28	Chesler4	tail suspension test	(none)	average latency to first immobility episode, 5 min test [s] TS_latency_immobile 47028	DO population w/par	9	both	12-16wks	N=8

Chesler4:29	Chesler4	body weight	(none)	body weight at dissection [g] bw 47029	DO population w/par	9	both	12-16wks	N=8

Chesler4: Behavioral and nociception traits in a Diversity Outbred (DO) mouse population and 8 founder inbred strains (2013)

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