Graham3: Baseline T cell immune phenotypes in males of 104 F1 hybrid lines of Collaborative Cross strains of mice (CC RIX) predict virologic and disease control upon SARS-CoV infection (2021)

Graham JB, Baric RS, Lund JM   With: Swarts JL, Leist SR, Schäfer A, Menachery VD, Gralinski LE, Jeng S, Miller DR, Mooney MA, McWeeney SK, Ferris MT, Pardo-Manuel de Villena F, Heise MT

         
Graham3 downloads
• Download Graham3 project data set     animal data, as uploaded
• Download Graham3 animal data matrix     with factor-related expansion applied as necessary
• Download Graham3 strain means, SD, N, etc.     one row per strain/sex/measure
Investigators Jessica B Graham       Fred Hutchinson Cancer Research Center,  Seattle, WA
Ralph S Baric       University of North Carolina,  Chapel Hill, NC
Jennifer M Lund       Fred Hutchinson Cancer Research Center,  Seattle, WA
Participants Swarts JL, Leist SR, Schäfer A, Menachery VD, Gralinski LE, Jeng S, Miller DR, Mooney MA, McWeeney SK, Ferris MT, Pardo-Manuel de Villena F, Heise MT
ContactJessica B Graham     jgraham@fredhutch.org
Part of a series: • Graham2 - SARS-CoV infection of females   see Graham2
• Graham3 - Baseline T cell signatures in males (no infection)   (this project) Graham3
Funding Provided ByNIH AI100625
Project type Phenotype strain survey data set
MPD identifiersGraham3     MPD:1059
Data changelog No updates/corrections.       Initial release date: 04/2021.
Formatted citation
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This study used a screen of genetically diverse mice (F1 hybrids of Collaborative Cross strains of mice (CC RIX)) infected with mouse-adapted SARS-CoV in combination with comprehensive pre-infection immunophenotyping to identify baseline circulating immune correlates of severe virologic and clinical outcomes upon SARS-CoV infection. Eight to ten week old female CC RIX mice were infected intranasally and monitored for death, weight loss, and lung viral loads (data from this part of the study are in Graham2). Age-matched, uninfected male CC RIX mice were used to assess baseline steady-state splenic T cell numbers (data from this part of the study are in this project, Graham3).

Experimental groups in this study:
• Baseline    

Procedures conducted:
• immune cell quantification  Baseline splenic T cell subsets.

Mice: CC RIX   104 strains   ♂   age 8-10wks   1 experimental group