Graham2: SARS-CoV infection in females of 117 F1 hybrid lines of Collaborative Cross strains of mice (CC RIX) (2021)

Graham JB, Swarts JL, Leist SR, Schäfer A, Menachery VD, Gralinski LE, Jeng S, Miller DR, Mooney MA, McWeeney SK, Ferris MT, Pardo-Manuel de Villena F, Heise MT, Baric RS, Lund JM. Baseline T cell immune phenotypes predict virologic and disease control upon SARS-CoV infection in Collaborative Cross mice. PLoS Pathog. 2021 Jan;17(1):e1009287. doi: 10.1371/journal.ppat.1009287. Epub 2021 Jan 29.   PubMed 33513210     FullText


         
This study used a screen of genetically diverse mice (F1 hybrids of Collaborative Cross strains of mice (CC RIX)) infected with mouse-adapted SARS-CoV in combination with comprehensive pre-infection immunophenotyping to identify baseline circulating immune correlates of severe virologic and clinical outcomes upon SARS-CoV infection. Eight to ten week old female CC RIX mice were infected intranasally and monitored for death, weight loss, and lung viral loads (data from this part of the study are in this project, Graham2). Age-matched, uninfected male CC RIX mice were used to assess baseline steady-state splenic T cell numbers (data from this part of the study are in Graham3).

Experimental groups in this study:
• SARS-CoV    

Procedures conducted:
• colony observation  Body weight loss and mortality.
• in vitro assay  Lung viral load (log10).

Graham2 downloads
• Download Graham2 project data set     strain means, as uploaded
• Download Graham2 strain means, SD, N, etc.     one row per strain/sex/measure
Investigators Jessica B Graham       Fred Hutchinson Cancer Research Center,  Seattle, WA
Ralph S Baric       University of North Carolina,  Chapel Hill, NC
Jennifer M Lund       Fred Hutchinson Cancer Research Center,  Seattle, WA
Participants Swarts JL, Leist SR, Schäfer A, Menachery VD, Gralinski LE, Jeng S, Miller DR, Mooney MA, McWeeney SK, Ferris MT, Pardo-Manuel de Villena F, Heise MT
ContactJessica B Graham     jgraham@fredhutch.org
Part of a series: • Graham2 - SARS-CoV infection of females   (this project) Graham2
• Graham3 - Baseline T cell signatures in males (no infection)   see Graham3
Funding Provided ByNIH AI100625
Project type Phenotype strain survey data set
MPD identifiersGraham2     MPD:1060
Data changelog No updates/corrections.       Initial release date: 04/2021.
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Mice: CC RIX   117 strains   ♀   age 8-10wks   1 experimental group