MPD: Data set: Richfield1

Richfield1: Behavioral, neurochemical, neuroanatomical, and neurotoxicological survey of the midbrain dopamine system in males of 15 inbred strains of mice (2003)

Mhyre TR, Chesler EJ, Thiruchelvam M, Lungu C, Cory-Slechta DA, Fry JD, Richfield EK. Heritability, correlations and in silico mapping of locomotor behavior and neurochemistry in inbred strains of mice. Genes Brain Behav. 2005 Jun;4(4):209-28. PubMed 15924554

Richfield1 downloads
• Download Richfield1 project data set animal data, as uploaded
• Download Richfield1 animal data matrix with factor-related expansion applied as necessary
• Download Richfield1 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Richfield1 measures:

MA:0000168 brain
MP:0001362 abnormal anxiety-related response
MP:0001392 abnormal locomotor behavior
MP:0001905 abnormal dopamine level
MP:0002206 abnormal CNS synaptic transmission
MP:0005322 abnormal serotonin level
VT:0001392 locomotor behavior trait
VT:0001905 dopamine amount
VT:0002206 CNS synaptic transmission trait
VT:0010484 serotonin amount
VT:0010716 anxiety-related behavior trait

Investigators	Eric K Richfield Environmental and Occupational Health Science Institute , Piscataway, NJ Timothy R Mhyre University of Rochester School of Medicine and Dentistry, Rochester, NY Deborah A Cory-Slechta Environmental and Occupational Health Science Institute , Piscataway, NJ Mona Thiruchelvam Environmental and Occupational Health Science Institute , Piscataway, NJ Elissa J Chesler The Jackson Laboratory, Bar Harbor, ME
Participants	Fry J, Lungu C
Contact	Eric K Richfield ekr@eohsi.rutgers.edu
Acknowledgements	Funding provided by NIH ES11839, DOD DAMD17-98-1-8628.
Project type	Phenotype strain survey data set
MPD identifiers	Richfield1 MPD:145
Data changelog	2 updates/corrections. Initial release date: 04/2008.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Locomotor behavioral parameters and neurotransmitter concentrations related to the midbrain dopamine system were assessed. The midbrain dopamine system is an important mediator of both normal and pathologic behaviors in many species, including humans. As such, it has received considerable attention regarding its roles in aspects of motor activity, attention, motivation/reward, and cognition.

Procedures conducted:

• open field test		Locomotor activity. 3 day trials. 45 min sessions.
• neurotransmitter quantification		Neurotransmitters. Molecular measures.

Mice: inbred 15 strains ♂ age 7-9wks 1 experimental group

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)

Richfield1:1	Richfield1	open field test	(none)	number of horizontal beam breaks, 45 min test [n] horiz 14501 day1 day2 day3 daily avg	inbred	15	m	7-9wks	N=11

Richfield1:4	Richfield1	open field test	(none)	ambulatory activity counts, 45 min test [n] amb 14504 day1 day2 day3 daily avg	inbred	15	m	7-9wks	N=11

Richfield1:7	Richfield1	open field test	(none)	number of vertical beam breaks, rearing, 45 min test [n] vert 14507 day1 day2 day3 daily avg	inbred	15	m	7-9wks	N=7

Richfield1:11	Richfield1	open field test	(none)	horizontal beam breaks per minute [n/min] horiz_rate 14511	inbred	15	m	7-9wks	N=11

Richfield1:12	Richfield1	open field test	(none)	long-term habituation, change in horizontal activity over days 1-3 [n/d] horiz_long_habit 14512	inbred	15	m	7-9wks	N=11

Richfield1:13	Richfield1	open field test	(none)	short-term habituation, change in horizontal activity over 45min, day 3 [n/min] horiz_short_habit 14513	inbred	15	m	7-9wks	N=11

Richfield1:15	Richfield1	open field test	(none)	rate of ambulatory activity per minute [n/min] amb_rate 14515	inbred	15	m	7-9wks	N=11

Richfield1:16	Richfield1	open field test	(none)	long-term habituation, change in ambulatory activity over days 1-3 [n/d] amb_long_habit 14516	inbred	15	m	7-9wks	N=11

Richfield1:17	Richfield1	open field test	(none)	short-term habituation, change in ambulatory activity over 45 min, day 3 [n/min] amb_short_habit 14517	inbred	15	m	7-9wks	N=11

Richfield1:19	Richfield1	open field test	(none)	vertical beam breaks per minute [n/min] vert_rate 14519	inbred	15	m	7-9wks	N=7

Richfield1:20	Richfield1	open field test	(none)	long-term habituation, change in vertical activity over days 1-3 [n/d] vert_long_habit 14520	inbred	15	m	7-9wks	N=7

Richfield1:21	Richfield1	open field test	(none)	short-term habituation, change in vertical activity over 45 min, day 3 [n/min] vert_short_habit 14521	inbred	15	m	7-9wks	N=7

Richfield1:22	Richfield1	neurotransmitter quantification	(none)	dopamine (DA), ng per mg total protein [ratio] DA 14522	inbred	15	m	7-9wks	N=10

Richfield1:23	Richfield1	neurotransmitter quantification	(none)	3,4-dihydroxyphenylacetic acid (DOPAC), ng per mg total protein [ratio] DOPAC 14523	inbred	15	m	7-9wks	N=10

Richfield1:24	Richfield1	neurotransmitter quantification	(none)	4-hydroxy-3-methoxyphenylacetic acid (HVA), ng per mg total protein [ratio] HVA 14524	inbred	15	m	7-9wks	N=10

Richfield1:25	Richfield1	neurotransmitter quantification	(none)	serotonin (5HT), ng per mg total protein [ratio] 5HT 14525	inbred	15	m	7-9wks	N=10

Richfield1:26	Richfield1	neurotransmitter quantification	(none)	dopamine turnover ([DOPAC]/[DA]) [ratio] DA_turnover1 14526	inbred	15	m	7-9wks	N=10

Richfield1:27	Richfield1	neurotransmitter quantification	(none)	dopamine turnover ([DOPAC] + [HVA]/[DA]) [ratio] DA_turnover2 14527	inbred	15	m	7-9wks	N=10