Threadgill1: Drug study: Toxicogenetic survey of susceptibility to acetaminophen-induced liver injury in males of 37 inbred strains of mice (2005)

Harrill AH, Watkins PB, Su S, Ross PK, Harbourt DE, Stylianou IM, Boorman GA, Russo MW, Sackler RS, Harris SC, Smith PC, Tennant R, Bogue M, Paigen K, Harris C, Contractor T, Wiltshire T, Rusyn I, Threadgill DW. Mouse population-guided resequencing reveals that variants in CD44 contribute to acetaminophen-induced liver injury in humans. Genome Res. 2009 Sep;19(9):1507-15. Epub 2009 May 5.   PubMed 19416960     FullText

Harrill AH, Ross PK, Gatti DM, Threadgill DW, Rusyn I. Population-based discovery of toxicogenomics biomarkers for hepatotoxicity using a laboratory strain diversity panel. Toxicol Sci. 2009 Jul;110(1):235-43. Epub 2009 May 6.   PubMed 19420014     FullText


       
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• Threadgill1 supplementary data
Participants David W Threadgill       Texas A&M University College Station, TX
Alison Harrill       University of Arkansas for Medical Sciences Little Rock, AR
Ivan Rusyn       Texas A&M University College Station, TX
With: Ross PK
ContactDavid W Threadgill     dwthreadgill@tamu.edu     Lab web site
Part of a series: • Phase 1   (this project) Threadgill1
• Phase 2   see Threadgill2
AcknowledgementsFunding provided by NIH ES07126, ES11391, ES10126, GM38149, RR00046, ES35513, ES25497, ES65406; US Environmental Protection Agency STAR-RD832720

For technical assistance and histology: Balletta L, Bradford B, Lodestro C, Lee D, Maki A, Kosyk O, Woods C, Clore E, Hodges N, Boorman G. For serum enzyme testing: Kim H and the Clinical Chemistry Core Facility (UNC).
Project type Phenotype strain survey data set
MPD identifiersThreadgill1     MPD:194
Data changelog 1 update/correction.       Initial release date: 02/2009.
Formatted citation
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An investigation of liver injury after administration of acetaminophen (N-acetyl-p-aminophenol; APAP) in various doses. The study involved 8 cohorts, including controls as well as animals treated with acetaminophen 30, 100, 200, 300, 600, 900, and 1200 mg/kg body weight. Animals were evaluated in these areas: blood and liver chemistry, liver function, and alterations in liver morphology. Data are presented here for selected cohorts and measurements.

Summary of procedures that were conducted
• metabolic panel Blood urea nitrogen, alanine transaminase, aspartate transaminase (serum), liver enzyme homeostasis. Acetaminophen (several doses) vs. control, several timepoints post-Rx. 18h fast.
• histopathology Liver histopathology, necrosis after acetaminophen vs. control. 18h fast.
• metabolite quantification Liver protein homeostasis. Acetaminophen vs. control. 18h fast.
• biomarker quantification Liver cytochrome isoforms. Acetaminophen vs. control. 18h fast.
• organ weights Liver weight after acetaminophen (several doses) vs. control. 18h fast.
Mice: inbred w/CC7   37 strains   ♂   age 8-17wks   8 cohorts