MPD: Data set: Palmer4

Palmer4: Behavioral traits in a panel of F1 hybrids involving 30 inbred strains and C57BL/6J males, where mice are +/- for the Cacna1c gene (2017)

Sittig LJ, Carbonetto P, Engel KA, Krauss KS, Barrios-Camacho CM, Palmer AA. Genetic Background Limits Generalizability of Genotype-Phenotype Relationships. Neuron. 2016 Sep 21;91(6):1253-9. doi: 10.1016/j.neuron.2016.08.013. Epub 2016 Sep 8. PubMed 27618673

Palmer4 downloads
• Download Palmer4 project data set animal data, as uploaded
• Download Palmer4 animal data matrix with factor-related expansion applied as necessary
• Download Palmer4 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Palmer4 measures:

MA:0000167 central nervous system
MP:0001259 abnormal body weight
MP:0001362 abnormal anxiety-related response
MP:0001392 abnormal locomotor behavior
MP:0001486 abnormal startle reflex
MP:0003360 abnormal depression-related behavior
MP:0013189 abnormal exploration in a new environment
VT:0001259 body mass
VT:0001392 locomotor behavior trait
VT:0010471 exploratory behavior trait
VT:0010716 anxiety-related behavior trait
VT:0010725 startle reflex trait
VT:0015015 depression-related behavior trait

Investigators	Laura J Sittig University of Chicago, Chicago, IL Abraham A Palmer University of California, San Diego, San Diego, CA
Participants	Carbonetto P, Engel KA, Krauss KS, Barrios-Camacho CM
Contact	Abraham A Palmer aapalmer@ucsd.edu Lab web site
Part of a series:	• Tcf7l2 +/+ see Palmer5 • Tcf7l2 +/- see Palmer3 • Cacna1c +/+ see Palmer6 • Cacna1c +/- (this project) Palmer4
Project type	Phenotype strain survey data set
MPD identifiers	Palmer4 MPD:593
Data changelog	No updates/corrections. Initial release date: 09/2017.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

F1 crosses were produced between male C57BL/6J mice heterozygous for the null allele of Cacna1c and wild-type females from 30 inbred laboratory strains. This gene has been associated with schizophrenia and bipolar disorder. The generalizability of Cacna1c +/- behavioral phenotypes was evaluated using these 30 genetic backgrounds. Data for +/+ and +/- littermates are summarized graphically here. Data were downloaded from https://github.com/pcarbo/neuron .

Procedures conducted:

• body weight
• light-dark box		Anxiety-related. 5 min test.
• open field test		Locomotor and exploratory activity, anxiety-related behavior, methamphetamine sensitivity. 30 min test.
• forced swim test		Immobility time. 6 min test.
• acoustic startle test		Acoustic startle response.

Mice: F1 Cacna1c 30 strains ♀♂ age 7-14wks 1 experimental group

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Palmer4:1	Palmer4	body weight	(none)	body weight [g] bw 59301	F1 Cacna1c	30	both	7-14wks	N=6

Palmer4:11	Palmer4	light-dark box	(none)	percentage of time spent in light vs dark side of apparatus [%] pct_light 59311	F1 Cacna1c	30	both	7-14wks	N=6

Palmer4:21	Palmer4	open field test	(none)	duration in center of arena on day 1 [s] center_d1 59321	F1 Cacna1c	30	both	7-14wks	N=6

Palmer4:22	Palmer4	open field test	(none)	total distance traveled on day 1, saline [cm] distance_d1 59322	F1 Cacna1c	30	both	7-14wks	N=6

Palmer4:23	Palmer4	open field test	(none)	total distance traveled on day 2, saline [cm] distance_d2 59323	F1 Cacna1c	30	both	7-14wks	N=6

Palmer4:24	Palmer4	open field test	methamphetamine	total distance traveled on day 3 (methamphetamine) minus total distance traveled on day 2 (saline) [cm] distance_d3_d2 59324	F1 Cacna1c	30	both	7-14wks	N=6

Palmer4:31	Palmer4	forced swim test	(none)	duration of immobility [s] immobility 59331	F1 Cacna1c	30	both	7-14wks	N=5

Palmer4:41	Palmer4	acoustic startle test	(none)	acoustic startle response (ASR) evoked by 120 dB sound pressure level [amplitude] ASR 59341	F1 Cacna1c	30	both	7-14wks	N=6