Palmer4: Behavioral traits in a panel of F1 hybrids involving 30 inbred strains where mice are +/- for the Cacna1c gene (2017)

Sittig LJ, Carbonetto P, Engel KA, Krauss KS, Barrios-Camacho CM, Palmer AA. Genetic Background Limits Generalizability of Genotype-Phenotype Relationships. Neuron. 2016 Sep 21;91(6):1253-9. doi: 10.1016/j.neuron.2016.08.013. Epub 2016 Sep 8.   PubMed 27618673  


       
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Participants Laura J Sittig       University of Chicago Chicago, IL
Abraham A Palmer       University of California, San Diego San Diego, CA
With: Carbonetto P, Engel KA, Krauss KS, Barrios-Camacho CM
ContactAbraham A Palmer     aapalmer@ucsd.edu     Lab web site
Part of a series: • Tcf7l2 +/+   see Palmer5
• Tcf7l2 +/-   see Palmer3
• Cacna1c +/+   see Palmer6
• Cacna1c +/-   (this project) Palmer4
Project type Phenotype strain survey data set
MPD identifiersPalmer4     MPD:593
Data changelog No updates/corrections.       Initial release date: 09/2017.
Formatted citation
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F1 crosses were produced between male C57BL/6J mice heterozygous for the null allele of Cacna1c and wild-type females from 30 inbred laboratory strains. This gene has been associated with schizophrenia and bipolar disorder. The generalizability of Cacna1c +/- behavioral phenotypes was evaluated using these 30 genetic backgrounds. Data for +/+ and +/- littermates are summarized graphically here. Data were downloaded from https://github.com/pcarbo/neuron .

Summary of procedures that were conducted
• body weight
• light-dark box Anxiety. 5 min test.
• open field test Methamphetamine sensitivity (exploratory activity). 30 min test.
• forced swim test Immobility time. 6 min test.
• acoustic startle test Acoustic startle response, prepulse inhibition.
Mice: F1 Cacna1c   30 strains   ♀♂   age 7-14wks   1 cohort

Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Palmer4 59301 body weight (none) body weight   [g]  bw F1 Cacna1c 30 both 7-14wks N=6
    Palmer4 59311 light-dark box (none) percentage of time spent in light vs dark side of apparatus   [%]  pct_light F1 Cacna1c 30 both 7-14wks N=6
    Palmer4 59321 open field test (none) duration in center of arena on day 1   [s]  center_d1 F1 Cacna1c 30 both 7-14wks N=6
    Palmer4 59322 open field test (none) total distance traveled on day 1 (saline)   [cm]  distance_d1 F1 Cacna1c 30 both 7-14wks N=6
    Palmer4 59323 open field test (none) total distance traveled on day 2 (saline)   [cm]  distance_d2 F1 Cacna1c 30 both 7-14wks N=6
    Palmer4 59324 open field test methamphetamine total distance traveled on day 3 (methamphetamine) minus total distance traveled on day 2 (saline)   [cm]  distance_d3_d2 F1 Cacna1c 30 both 7-14wks N=6
    Palmer4 59331 forced swim test (none) duration of immobility   [s]  immobility F1 Cacna1c 30 both 7-14wks N=5
    Palmer4 59341 acoustic startle test (none) acoustic startle response (ASR) evoked by 120 dB sound pressure level   [amplitude]  ASR F1 Cacna1c 30 both 7-14wks N=6
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