MPD: Data set: Brugnara1

Rivera A, Zee RY, Alper SL, Peters LL, Brugnara C. Strain-specific variations in cation content and transport in mouse erythrocytes. Physiol Genomics. 2013 May 1;45(9):343-50. doi: 10.1152/physiolgenomics.00143.2012. Epub 2013 Mar 12. PubMed 23482811

Procedures conducted:

• complete blood count		Hematology.
• ion transport assessment		Hematology red cell ion content, ion transport.

Brugnara1 downloads
• Download Brugnara1 project data set animal data, as uploaded
• Download Brugnara1 animal data matrix with factor-related expansion applied as necessary
• Download Brugnara1 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Brugnara1 measures:

MA:0000059 blood
MP:0000226 abnormal mean corpuscular volume
MP:0001589 abnormal mean corpuscular hemoglobin
MP:0004231 abnormal calcium ion homeostasis
MP:0005640 abnormal mean corpuscular hemoglobin concentration
MP:0011913 abnormal reticulocyte cell number
MP:0011978 abnormal potassium ion homeostasis
MP:0012373 abnormal erythrocyte magnesium ion content
MP:0012376 abnormal erythrocyte potassium ion content
MP:0012379 abnormal erythrocyte sodium ion content
MP:0012385 abnormal erythrocyte potassium:chloride symporter activity
MP:0012388 abnormal erythrocyte sodium:hydrogen antiporter activity
MP:0012391 abnormal erythrocyte sodium:potassium-exchanging ATPase activity
MP:0012394 abnormal erythrocyte calcium-activated potassium channel activity
MP:0012397 abnormal nucleated erythrocyte cell number
VT:0000248 erythrocyte size trait
VT:0001562 blood calcium amount
VT:0001588 blood hemoglobin amount
VT:0001771 blood magnesium amount
VT:0001776 blood sodium amount
VT:0002447 erythrocyte morphology trait
VT:0002668 blood potassium amount
VT:0003018 blood chloride amount
VT:0003135 reticulocyte quantity
VT:0004152 blood iron amount
VT:0010534 erythrocyte ion transport trait
VT:0010535 erythrocyte ion amount

Investigators	Carlo Brugnara Harvard Medical School, Boston, MA Luanne L Peters The Jackson Laboratory, Bar Harbor, ME
Participants	Rivera A, Zee RY, Alper SL
Contact	Carlo Brugnara Carlo.Brugnara@childrens.harvard.edu
Acknowledgements	Funding provided by NIH HL085480, HL081497, HL090632, CA34196
Project type	Phenotype strain survey data set
MPD identifiers	Brugnara1 MPD:474
Data changelog	No updates/corrections. Initial release date: 05/2013.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Brugnara1:1	Brugnara1	ion transport assessment	(none)	RBC sodium cation content (mmol/kg hemoglobin) [mmol/kg] sodium 47401	inbred	10	both	8wks	N=31

Brugnara1:2	Brugnara1	ion transport assessment	(none)	RBC potassium cation content (mmol/kg hemoglobin) [mmol/kg] potassium 47402	inbred	10	both	8wks	N=31

Brugnara1:3	Brugnara1	ion transport assessment	(none)	RBC magnesium cation content (mmol/kg hemoglobin) [mmol/kg] magnesium 47403	inbred	10	both	8wks	N=28

Brugnara1:4	Brugnara1	ion transport assessment	(none)	RBC potassium chloride cotransport (KCC; per 10¹³ cells) [mmol⋅h] K_Cl 47404	inbred	10	both	8wks	N=14

Brugnara1:5	Brugnara1	ion transport assessment	(none)	RBC sodium-potassium pump (Na-K; per 10¹³ cells) [mmol⋅h] Na_K_pump 47405	inbred	10	both	8wks	N=11

Brugnara1:6	Brugnara1	ion transport assessment	(none)	RBC sodium-hydrogen exchanger (NHE1; per 10¹³ cells) [mmol⋅h] Na_H_exchange 47406	inbred	10	both	8wks	N=10

Brugnara1:7	Brugnara1	ion transport assessment	(none)	RBC calcium-activated potassium channel (KCNN4) [mmol/L⋅min] gardos_channel 47407	inbred	10	both	8wks	N=11

Brugnara1:8	Brugnara1	complete blood count	(none)	mean RBC corpuscular volume (MCV) [fL] MCV 47408	inbred	10	both	8wks	N=31

Brugnara1:9	Brugnara1	complete blood count	(none)	RBC corpuscular hemoglobin concentration mean (CHCM) [g/dL] CHCM 47409	inbred	10	both	8wks	N=32

Brugnara1:10	Brugnara1	complete blood count	(none)	mean RBC corpuscular hemoglobin conent (MCH) [pg] MCH 47410	inbred	10	both	8wks	N=31

Brugnara1:11	Brugnara1	complete blood count	(none)	reticulocyte differential (Retic; percentage of total RBC) [%] retic 47411	inbred	10	both	8wks	N=30

Brugnara1: Erythrocyte cation content and transport in 10 inbred strains of mice (2013)