MPD: Data set: Gould2

Portugal GS, Wilkinson DS, Kenney JW, Sullivan C, Gould TJ. Strain-dependent Effects of Acute, Chronic, and Withdrawal from Chronic Nicotine on Fear Conditioning. Behav Genet. 2012 Jan;42(1):133-50. Epub 2011 Aug 6. PubMed 21822688

Gould2 downloads
• Download Gould2 project data set one row per animal, one column per trait
• Download Gould2 strain means, SD, N, etc. one row per strain/sex/measure
• Gould2 supplementary data

Ontology terms mapped to Gould2 measures:
MA:0002501 plasma
MP:0001362 abnormal anxiety-related response
MP:0001440 abnormal grooming behavior
MP:0001454 abnormal cued conditioning behavior
MP:0001469 abnormal contextual conditioning behavior
MP:0002062 abnormal associative learning
MP:0003106 abnormal fear-related response
MP:0003492 abnormal involuntary movement
MP:0008872 abnormal physiological response to xenobiotic
MP:0012695 abnormal nicotine metabolism
MP:0013189 abnormal exploration in a new environment
MP:0020160 abnormal behavioral response to nicotine
VT:0001412 scratching trait
VT:0001440 grooming behavior trait
VT:0002121 metabolism trait
VT:0003492 involuntary movement trait
VT:0010471 exploratory behavior trait
VT:0010488 response to addictive substance trait
VT:0010527 associative learning trait
VT:0010529 contextual conditioning behavior trait
VT:0010530 cued conditioning behavior trait
VT:0010715 fear-related behavior trait
VT:0010716 anxiety-related behavior trait
VT:0010721 response to nicotine trait

Participants	George S Portugal Columbia University, New York, NY Thomas J Gould Temple University, Philadelphia, PA With: Wilkinson DS, Kenney JW, Sullivan C
Contact	Thomas J Gould tgould@temple.edu Lab web site
Part of a series:	• nicotine acute and chronic study groups (this project) Gould2 • nicotine withdrawal study group see Gould3
Acknowledgements	Funding provided by NIH DA017949, DA024787, CA143187
Project type	Phenotype strain survey data set
MPD identifiers	Gould2 MPD:471
Data changelog	No updates/corrections. Initial release date: 05/2013.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Acute group cohorts include saline controls and nicotine dose groups of 0.045, 0.09, and 0.18 mg/kg. Chronic group cohorts include saline controls and nicotine dose of 12 mg/kg/d administered for 14 days. Plasma assay and fear conditioning assays were done for both acute and chronic groups. EPM and somatic trait observation were done for the chronic group only.

Summary of procedures that were conducted
• drug and metabolite quantification	Amount of nicotine and cotinine in blood plasma after nicotine doses. High and lower doses.
• behavior observation	Observed measures of anxiety. Nicotine (high dose) vs. control. 20 min period.
• elevated plus maze	Measures of anxiety. Nicotine (high dose) vs. control. 5 min test.
• fear conditioning test	Contextual fear, cued fear. Nicotine (high and several lower doses) vs. control.

Data set	MPD ID	Procedure / Protocol	Drug or Challenge	Phenotype Measure	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Gould2	47101	drug and metabolite quantification	nicotine	plasma nicotine [ng/mL] acute 0.09mg dose chronic 6.3mg dose	inbred	8	m	8-12wks	N=10

Gould2	47105	drug and metabolite quantification	nicotine	plasma cotinine [ng/mL] acute 0.09mg dose chronic 6.3mg dose	inbred	8	m	8-12wks	N=10

Gould2	47111	behavior observation	nicotine	chronic group, grooming [n] control nicotine	inbred	8	m	8-12wks	N=10

Gould2	47113	behavior observation	nicotine	chronic group, scratching [n] control nicotine	inbred	8	m	8-12wks	N=10

Gould2	47115	behavior observation	nicotine	chronic group, jumping [n] control nicotine	inbred	8	m	8-12wks	N=10

Gould2	47121	elevated plus maze	nicotine	chronic group, time in closed arms [%] control nicotine	inbred	8	m	8-12wks	N=9

Gould2	47123	elevated plus maze	nicotine	chronic group, time in open arms [%] control nicotine	inbred	8	m	8-12wks	N=9

Gould2	47125	elevated plus maze	nicotine	chronic group, time in center [%] control nicotine	inbred	8	m	8-12wks	N=9

Gould2	47131	fear conditioning test	nicotine	acute group, percent immobility in training chamber after first stimulus pairing [%] control nicotine dose (mg): 0.045 0.09 0.18	inbred	8	m	8-12wks	N=11

Gould2	47136	fear conditioning test	nicotine	acute group, percent immobility in training chamber, 24h after fear conditioning [%] control nicotine dose (mg): 0.045 0.09 0.18	inbred	8	m	8-12wks	N=11

Gould2	47141	fear conditioning test	nicotine	acute group, percent immobility in altered context chamber, pre-cue [%] control nicotine dose (mg): 0.045 0.09 0.18	inbred	8	m	8-12wks	N=11

Gould2	47146	fear conditioning test	nicotine	acute group, percent immobility in altered context chamber, cue [%] control nicotine dose (mg): 0.045 0.09 0.18	inbred	8	m	8-12wks	N=11

Gould2	47151	fear conditioning test	nicotine	chronic group, conditioning: immediate fear [%] control nicotine	inbred	8	m	8-12wks	N=10

Gould2	47156	fear conditioning test	nicotine	chronic group, conditioning: contextual fear [%] control nicotine	inbred	8	m	8-12wks	N=10

Gould2	47161	fear conditioning test	nicotine	chronic group, cued fear: pre-cue [%] control nicotine	inbred	8	m	8-12wks	N=10

Gould2	47166	fear conditioning test	nicotine	chronic group, cued fear: cue [%] control nicotine	inbred	8	m	8-12wks	N=10

Gould2: Drug study: Effects of acute and chronic nicotine on fear conditioning and other behaviors in males of 8 inbred mouse strains (2011)