MPD: Data set: Gould2

Portugal GS, Wilkinson DS, Kenney JW, Sullivan C, Gould TJ. Strain-dependent Effects of Acute, Chronic, and Withdrawal from Chronic Nicotine on Fear Conditioning. Behav Genet. 2012 Jan;42(1):133-50. Epub 2011 Aug 6. PubMed 21822688

Acute group cohorts include saline controls and nicotine dose groups of 0.045, 0.09, and 0.18 mg/kg. Chronic group cohorts include saline controls and nicotine dose of 12 mg/kg/d administered for 14 days. Plasma assay and fear conditioning assays were done for both acute and chronic groups. EPM and somatic trait observation were done for the chronic group only.

Experimental groups in this study:
• None • None • None • None • None • None • None • None

Procedures conducted:

• drug and metabolite quantification		Amount of nicotine and cotinine in blood plasma after nicotine doses. High and lower doses.
• behavior observation		Observed measures of anxiety. Nicotine (high dose) vs. control. 20 min period.
• elevated plus maze		Measures of anxiety. Nicotine (high dose) vs. control. 5 min test.
• fear conditioning test		Contextual fear, cued fear. Nicotine (high and several lower doses) vs. control.

Gould2 downloads
• Download Gould2 project data set animal data, as uploaded
• Download Gould2 animal data matrix with factor-related expansion applied as necessary
• Download Gould2 strain means, SD, N, etc. one row per strain/sex/measure
• Gould2 supplementary data

Ontology terms mapped to Gould2 measures:

MA:0002501 plasma
MP:0001362 abnormal anxiety-related response
MP:0001440 abnormal grooming behavior
MP:0001454 abnormal cued conditioning behavior
MP:0001469 abnormal contextual conditioning behavior
MP:0002062 abnormal associative learning
MP:0003106 abnormal fear-related response
MP:0003492 abnormal involuntary movement
MP:0008872 abnormal physiological response to xenobiotic
MP:0012695 abnormal nicotine metabolism
MP:0013189 abnormal exploration in a new environment
MP:0020160 abnormal behavioral response to nicotine
VT:0001412 scratching trait
VT:0001440 grooming behavior trait
VT:0002121 metabolism trait
VT:0003492 involuntary movement trait
VT:0010471 exploratory behavior trait
VT:0010527 associative learning trait
VT:0010529 contextual fear conditioning behavior trait
VT:0010530 cued fear conditioning behavior trait
VT:0010715 fear-related behavior trait
VT:0010716 anxiety-related behavior trait
VT:0010721 response to nicotine trait

Investigators	George S Portugal Columbia University, New York, NY Thomas J Gould Temple University, Philadelphia, PA
Participants	Wilkinson DS, Kenney JW, Sullivan C
Contact	Thomas J Gould tgould@temple.edu Lab web site
Part of a series:	• nicotine acute and chronic study groups (this project) Gould2 • nicotine withdrawal study group see Gould3
Acknowledgements	Funding provided by NIH DA017949, DA024787, CA143187
Project type	Phenotype strain survey data set
MPD identifiers	Gould2 MPD:471
Data changelog	No updates/corrections. Initial release date: 05/2013.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Gould2:1	Gould2	drug and metabolite quantification	nicotine	plasma nicotine [ng/mL] nicotine 47101 acute 0.09mg dose chronic 6.3mg dose	inbred	8	m	8-12wks	N=10

Gould2:3	Gould2	drug and metabolite quantification	nicotine	plasma cotinine [ng/mL] cotinine 47105 acute 0.09mg dose chronic 6.3mg dose	inbred	8	m	8-12wks	N=10

Gould2:5	Gould2	behavior observation	nicotine	chronic group, grooming [n] grooming 47111 control nicotine	inbred	8	m	8-12wks	N=10

Gould2:7	Gould2	behavior observation	nicotine	chronic group, scratching [n] scratching 47113 control nicotine	inbred	8	m	8-12wks	N=10

Gould2:9	Gould2	behavior observation	nicotine	chronic group, jumping [n] jumping 47115 control nicotine	inbred	8	m	8-12wks	N=10

Gould2:11	Gould2	elevated plus maze	nicotine	chronic group, time in closed arms [%] EPM_pct_closed 47121 control nicotine	inbred	8	m	8-12wks	N=9

Gould2:13	Gould2	elevated plus maze	nicotine	chronic group, time in open arms [%] EPM_pct_open 47123 control nicotine	inbred	8	m	8-12wks	N=9

Gould2:15	Gould2	elevated plus maze	nicotine	chronic group, time in center [%] EPM_pct_center 47125 control nicotine	inbred	8	m	8-12wks	N=9

Gould2:17	Gould2	fear conditioning test	nicotine	acute group, percent immobility in training chamber after first stimulus pairing [%] FC_immediate_acu 47131 dose (mg): control 0.045 0.09 0.18	inbred	8	m	8-12wks	N=11

Gould2:21	Gould2	fear conditioning test	nicotine	acute group, percent immobility in training chamber, 24h after fear conditioning [%] FC_context_acu 47136 dose (mg): control 0.045 0.09 0.18	inbred	8	m	8-12wks	N=11

Gould2:25	Gould2	fear conditioning test	nicotine	acute group, percent immobility in altered context chamber, pre-cue [%] FC_preCS_acu 47141 dose (mg): control 0.045 0.09 0.18	inbred	8	m	8-12wks	N=11

Gould2:29	Gould2	fear conditioning test	nicotine	acute group, percent immobility in altered context chamber, cue [%] FC_CS_acu 47146 dose (mg) control 0.045 0.09 0.18	inbred	8	m	8-12wks	N=11

Gould2:33	Gould2	fear conditioning test	nicotine	chronic group, conditioning: immediate fear [%] FC_immediate_chr 47151 control nicotine	inbred	8	m	8-12wks	N=10

Gould2:35	Gould2	fear conditioning test	nicotine	chronic group, conditioning: contextual fear [%] FC_context_chr 47156 control nicotine	inbred	8	m	8-12wks	N=10

Gould2:37	Gould2	fear conditioning test	nicotine	chronic group, cued fear: pre-cue [%] FC_preCS_chr 47161 control nicotine	inbred	8	m	8-12wks	N=10

Gould2:39	Gould2	fear conditioning test	nicotine	chronic group, cued fear: cue [%] FC_CS_chr 47166 control nicotine	inbred	8	m	8-12wks	N=10

Gould2: Drug study: Effects of acute and chronic nicotine on fear conditioning and other behaviors in males of 8 inbred mouse strains (2011)