Participants	George S Portugal       Columbia University New York, NY Thomas J Gould       Temple University Philadelphia, PA With: Wilkinson DS, Kenney JW, Sullivan C
Contact	Thomas J Gould     tgould@temple.edu     Lab web site
Part of a series:	• nicotine acute and chronic study groups   (this project) Gould2 • nicotine withdrawal study group   see Gould3
Acknowledgements	Funding provided by NIH DA017949, DA024787, CA143187
Project type	Phenotype strain survey data set
MPD identifiers	Gould2     MPD:471
Data changelog	No updates/corrections.       Initial release date: 05/2013.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Acute group cohorts include saline controls and nicotine dose groups of 0.045, 0.09, and 0.18 mg/kg. Chronic group cohorts include saline controls and nicotine dose of 12 mg/kg/d administered for 14 days. Plasma assay and fear conditioning assays were done for both acute and chronic groups. EPM and somatic trait observation were done for the chronic group only.

Summary of procedures that were conducted
• drug and metabolite quantification	Amount of nicotine and cotinine in blood plasma after nicotine doses. High and lower doses.
• behavior observation	Observed measures of anxiety. Nicotine (high dose) vs. control. 20 min period.
• elevated plus maze	Measures of anxiety. Nicotine (high dose) vs. control. 5 min test.
• fear conditioning test	Contextual fear, cued fear. Nicotine (high and several lower doses) vs. control.