Gould2: Drug study: Effects of acute and chronic nicotine on fear conditioning and other behaviors in males of 8 inbred mouse strains (2011)

Portugal GS, Wilkinson DS, Kenney JW, Sullivan C, Gould TJ. Strain-dependent Effects of Acute, Chronic, and Withdrawal from Chronic Nicotine on Fear Conditioning. Behav Genet. 2012 Jan;42(1):133-50. Epub 2011 Aug 6.   PubMed 21822688  


       
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Participants George S Portugal       Columbia University New York, NY
Thomas J Gould       Temple University Philadelphia, PA
With: Wilkinson DS, Kenney JW, Sullivan C
ContactThomas J Gould     tgould@temple.edu     Lab web site
Part of a series: • nicotine acute and chronic study groups   (this project) Gould2
• nicotine withdrawal study group   see Gould3
AcknowledgementsFunding provided by NIH DA017949, DA024787, CA143187
Project type Phenotype strain survey data set
MPD identifiersGould2     MPD:471
Data changelog No updates/corrections.       Initial release date: 05/2013.
Formatted citation
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Acute group cohorts include saline controls and nicotine dose groups of 0.045, 0.09, and 0.18 mg/kg. Chronic group cohorts include saline controls and nicotine dose of 12 mg/kg/d administered for 14 days. Plasma assay and fear conditioning assays were done for both acute and chronic groups. EPM and somatic trait observation were done for the chronic group only.



Summary of procedures that were conducted
• drug and metabolite quantification Amount of nicotine and cotinine in blood plasma after nicotine doses. High and lower doses.
• behavior observation Observed measures of anxiety. Nicotine (high dose) vs. control. 20 min period.
• elevated plus maze Measures of anxiety. Nicotine (high dose) vs. control. 5 min test.
• fear conditioning test Contextual fear, cued fear. Nicotine (high and several lower doses) vs. control.
Mice: inbred   8 strains   ♂   age 8-12wks   8 cohorts

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Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Gould2 47101 drug and metabolite quantification nicotine plasma nicotine   [ng/mL]     acute 0.09mg dose    chronic 6.3mg dose inbred 8 m 8-12wks N=10
    Gould2 47105 drug and metabolite quantification nicotine plasma cotinine   [ng/mL]     acute 0.09mg dose    chronic 6.3mg dose inbred 8 m 8-12wks N=10
    Gould2 47111 behavior observation nicotine chronic group: grooming   [n]     control    nicotine inbred 8 m 8-12wks N=10
    Gould2 47113 behavior observation nicotine chronic group: scratching   [n]     control    nicotine inbred 8 m 8-12wks N=10
    Gould2 47115 behavior observation nicotine chronic group: jumping   [n]     control    nicotine inbred 8 m 8-12wks N=10
    Gould2 47121 elevated plus maze nicotine chronic group: time in closed arms   [%]     control    nicotine inbred 8 m 8-12wks N=9
    Gould2 47123 elevated plus maze nicotine chronic group: time in open arms   [%]     control    nicotine inbred 8 m 8-12wks N=9
    Gould2 47125 elevated plus maze nicotine chronic group: time in center   [%]     control    nicotine inbred 8 m 8-12wks N=9
    Gould2 47131 fear conditioning test nicotine acute group, percent immobility in training chamber after first stimulus pairing   [%]     control    nicotine dose (mg):    0.045    0.09    0.18 inbred 8 m 8-12wks N=11
    Gould2 47136 fear conditioning test nicotine acute group, percent immobility in training chamber, 24h after fear conditioning   [%]     control    nicotine dose (mg):    0.045    0.09    0.18 inbred 8 m 8-12wks N=11
    Gould2 47141 fear conditioning test nicotine acute group, percent immobility in altered context chamber, pre-cue   [%]     control    nicotine dose (mg):    0.045    0.09    0.18 inbred 8 m 8-12wks N=11
    Gould2 47146 fear conditioning test nicotine acute group, percent immobility in altered context chamber, cue   [%]     control    nicotine dose (mg):    0.045    0.09    0.18 inbred 8 m 8-12wks N=11
    Gould2 47151 fear conditioning test nicotine chronic group, conditioning: immediate fear   [%]     control    nicotine inbred 8 m 8-12wks N=10
    Gould2 47156 fear conditioning test nicotine chronic group, conditioning: contextual fear   [%]     control    nicotine inbred 8 m 8-12wks N=10
    Gould2 47161 fear conditioning test nicotine chronic group, cued fear: pre-cue   [%]     control    nicotine inbred 8 m 8-12wks N=10
    Gould2 47166 fear conditioning test nicotine chronic group, cued fear: cue   [%]     control    nicotine inbred 8 m 8-12wks N=10
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