Shorter5: Male reproduction in 28 F1 hybrids of Collaborative Cross strains of mice (RIX): Organ weights and sperm count, motility, and morphology (2020)

Shorter JR, O'Brien DA, Pardo-Manuel de Villena F   With: Pan W, Odet F, Aylor DL, Kao C-Y, Fu C-P, Greenstein S, Bell TA, Stevans AM, Patel S, Cates SE, Shaw GD, MIller DR, McMillian L

         
Male reproductive parameters were assessed. This project contains data for organ weights and sperm count, motility, and morphology while Shorter7 contains Computer Assisted Sperm Analysis (CASA) data.

Procedures conducted:
• body weight 
• organ weights  Male reproductive organ weights. Testes, seminal vesicles, epididymis + vas deferens.
• sperm count  Sperm count. Hemocytometer.
• microscopy  Sperm motility
• histopathology  Male reproductive histopathology. Percent normal, abnormal sperm.

Shorter5 downloads
• Download Shorter5 project data set     animal data, as uploaded
• Download Shorter5 animal data matrix     with factor-related expansion applied as necessary
• Download Shorter5 strain means, SD, N, etc.     one row per strain/sex/measure
• Download Shorter5 supplementary file, Shorter5_Supp_Data_F2s.xlsx     Shorter5 Supplemental Data for CC F2s
Investigators John R Shorter       University of North Carolina,  Chapel Hill, NC
Deborah A O'Brien       University of North Carolina,  Chapel Hill, NC
Fernando Pardo-Manuel de Villena       University of North Carolina,  Chapel Hill, NC
Participants Pan W, Odet F, Aylor DL, Kao C-Y, Fu C-P, Greenstein S, Bell TA, Stevans AM, Patel S, Cates SE, Shaw GD, MIller DR, McMillian L
ContactFernando Pardo-Manuel de Villena     fernando@med.unc.edu     Lab web site
Part of a series: • Organ weights, sperm count and morphology   (this project) Shorter5
• Sperm motility   see Shorter7
AcknowledgementsFunding provided by NIH HD065024; Eunice Kennedy Shriver National Institute of Child Health and Human Development. We would like to thank Lisa Branstetter, T. Justin Gooch, Stephanie Hansen, Ginger Shaw, Jennifer Shockley and Jason Spence for mouse work.
Project type Phenotype strain survey data set
MPD identifiersShorter5     MPD:655
Data changelog No updates/corrections.       Initial release date: 06/2020.
Formatted citation
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Mice: CC RIX   28 strains   ♂   age 7-44wks   1 experimental group

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Data set
Procedure / Protocol
Treatment
Phenotype Measure / Variable (click to view)
Panel
No. of Strains
Sex
Age
Sample Size (Avg)
    Shorter5 body weight (none) body weight   [g]  bw   110118     CC RIX 28 m 7-44wks N=4
    Shorter5 organ weights (none) testis weight   [g]  testis   110143     left    right CC RIX 28 m 7-44wks N=4
    Shorter5 organ weights (none) testis weight mean   [g]  testis_mean   110121     CC RIX 28 m 7-44wks N=4
    Shorter5 organ weights (none) testis weight as percentage of body weight   [%]  testis_pct_bw   110122     CC RIX 28 m 7-44wks N=4
    Shorter5 organ weights (none) epididymis plus vas deferens weight   [g]  epidid_vd   110145     left    right CC RIX 28 m 7-44wks N=4
    Shorter5 organ weights (none) epididymis plus vas deferens weight mean   [g]  epidid_vd_mean   110125     CC RIX 28 m 7-44wks N=4
    Shorter5 organ weights (none) epididymis plus vas deferens weight as percentage of body weight   [%]  epidid_vd_pct_bw   110126     CC RIX 28 m 7-44wks N=4
    Shorter5 organ weights (none) seminal vesicles weight total   [g]  semin_vesi_tot   110127     CC RIX 28 m 7-44wks N=4
    Shorter5 organ weights (none) seminal vesicles weight as percentage of body weight   [%]  semin_vesi_pct_bw   110128     CC RIX 28 m 7-44wks N=4
    Shorter5 sperm count (none) sperm count per mouse (x 106)   [n]  sperm_count   110129     CC RIX 28 m 7-44wks N=4
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