Shorter5 project protocol

Male reproduction in 28 F1 hybrids of Collaborative Cross strains of mice (RIX): Organ weights and sperm count, motility, and morphology   (2020)

Shorter JR, O'Brien DA, Pardo-Manuel de Villena F
With: Pan W, Odet F, Aylor DL, Kao C-Y, Fu C-P, Greenstein S, Bell TA, Stevans AM, Patel S, Cates SE, Shaw GD, MIller DR, McMillian L




Project protocol - Contents

Workflow and sampling

Step Description Equipment Data collected
1 Mice euthanized and weighed Scales Body weight
2 Reproductive organs harvested and weighed Scales Testis, epididymis with attached vas deferens, and seminal vesicle weights
3 Sperm harvested from right cauda epididymis and counted Hemocytometer Sperm counts
4 Sperm harvested from left cauda epididymis for in vitro capacitation study on sperm motility (see Shorter7) - -
5 Sperm from left cauda epididymis prepared to assess morphology Microscope Sperm morphology

Procedures

General Information for All Procedures: See Shorter7 for assessing sperm motility