MPD: Data set: Neuner1

Neuner1: Motor function, spatial learning and memory function, anxiety-related behaviors, and beta-amyloid levels in Alzheimer’s Disease mouse models (AD-BXD) (2019)

Neuner SM, Heuer SE, Huentelman MJ, O'Connell KMS, Kaczorowski CC. Harnessing Genetic Complexity to Enhance Translatability of Alzheimer's Disease Mouse Models: A Path toward Precision Medicine. Neuron. 2019 Feb 6;101(3):399-411.e5. doi: 10.1016/j.neuron.2018.11.040. Epub 2018 Dec 27. PubMed 30595332

Neuner SM, Heuer SE, Zhang JG, Philip VM, Kaczorowski CC. Identification of Pre-symptomatic Gene Signatures That Predict Resilience to Cognitive Decline in the Genetically Diverse AD-BXD Model. Front Genet. 2019 Feb 6;10:35. doi: 10.3389/fgene.2019.00035. eCollection 2019. PubMed 30787942 FullText

Procedures conducted:

• body weight		Measured every 2 mo, starting at age 2 mo to 16 mo.
• biomarker quantification		Temporal cortex amyloid level, age 6 and 14 mo.
• Y-maze		Spontaneous alternation and other parameters measured every 2 mo, from age 2 to 16 mo.
• balance beam		Time to cross narrow beam, age 6 and 14 mo.
• inclined screen test		Time to right body, age 6 and 14 mo.
• grip strength		Grip strength across 3 trials, age 6 mo and 14 mo.
• behavior observation		Sensorimotor composite score, age 6 mo and 14 mo.
• elevated plus maze		Distance traveled and arm entries, age 6 mo and 14 mo.
• fear conditioning test		Time spent freezing, age 6 mo and 14 mo.

Neuner1 downloads
• Download Neuner1 project data set animal data, as uploaded
• Download Neuner1 animal data matrix with factor-related expansion applied as necessary
• Download Neuner1 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Neuner1 measures:

MA:0000059 blood
MA:0000615 forelimb muscle
MA:0000941 temporal cortex
MP:0000188 abnormal circulating glucose level
MP:0001362 abnormal anxiety-related response
MP:0001469 abnormal contextual conditioning behavior
MP:0001515 abnormal grip strength
MP:0001516 abnormal motor coordination/balance
MP:0003106 abnormal fear-related response
MP:0003107 abnormal response to novelty
MP:0003329 amyloid beta deposits
MP:0013189 abnormal exploration in a new environment
VT:0000188 blood glucose amount
VT:0001259 body mass
VT:0001516 motor coordination/balance trait
VT:0004262 physical strength trait
VT:0010080 skeletal muscle physiology trait
VT:0010459 behavioral response to novel environment trait
VT:0010471 exploratory behavior trait
VT:0010529 contextual fear conditioning behavior trait
VT:0010585 brain molecular composition trait
VT:0010715 fear-related behavior trait
VT:0010716 anxiety-related behavior trait

Investigators	Sarah M Neuner The Jackson Laboratory, Bar Harbor, ME Catherine C Kaczorowski The Jackson Laboratory, Bar Harbor, ME
Participants	Heuer SE, Huentelman MJ, O’Connell KMS, Zhang JG, Philip VM
Contact	Catherine C Kaczorowski Catherine.Kaczorowski@jax.org Lab web site
Acknowledgements	Funding provided by NIH AG057914, AG054180, AG050357, AG059778, DK102918; BrightFocus Foundation A2016397S
Project type	Phenotype strain survey data set
MPD identifiers	Neuner1 MPD:659
Data changelog	No updates/corrections. Initial release date: 11/2019.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Mice: F1 AD-BXD 55 strains ♀♂ age 7-65wks 1 experimental group

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Neuner1:1	Neuner1	body weight	(none)	body weight [g] bw 65901 2 mo 4 mo 6 mo 8 mo 10 mo 12 mo 14 mo 16 mo	F1 AD-BXD	55	both	8-65wks	N=2

Neuner1:9	Neuner1	biomarker quantification	(none)	temporal cortex amyloid level (ng/mg total protein) [ng/mg] amyloid 65909 6 mo 14 mo	F1 AD-BXD	23	both	23-57wks	N=2

Neuner1:11	Neuner1	metabolic panel	(none)	blood glucose level [mg/dL] glucose 65911 6 mo 14 mo	F1 AD-BXD	55	both	23-57wks	N=6

Neuner1:13	Neuner1	Y-maze	(none)	percentage spontaneous alternation in Y-maze [%] Ymaze_spon_alt 65913 2 mo 4 mo 6 mo 8 mo 10 mo 12 mo 14 mo 16 mo	F1 AD-BXD	55	both	7-65wks	N=2

Neuner1:21	Neuner1	Y-maze	(none)	age at onset of working memory deficits (age at which spontaneous alternation percentage dropped below 50%, past 6 mo) [n] Ymaze_age_onset 65921	F1 AD-BXD	53	both	23-65wks	N=4

Neuner1:22	Neuner1	Y-maze	(none)	distance traveled in Y-maze [m] Ymaze_dist 65922 2 mo 4 mo 6 mo 8 mo 10 mo 12 mo 14 mo 16 mo	F1 AD-BXD	55	both	8-65wks	N=2

Neuner1:30	Neuner1	Y-maze	(none)	number of arm entries in Y-maze [n] Ymaze_ent 65930 2 mo 4 mo 6 mo 8 mo 10 mo 12 mo 14 mo 16 mo	F1 AD-BXD	55	both	8-65wks	N=2

Neuner1:38	Neuner1	Y-maze	(none)	percentage of alternating arm returns in Y-maze [%] Ymaze_returns 65938 2 mo 4 mo 6 mo 8 mo 10 mo 12 mo 14 mo 16 mo	F1 AD-BXD	55	both	8-65wks	N=2

Neuner1:46	Neuner1	balance beam	(none)	mean time to cross narrow beam, 3 min test, across 3 trials [s] beam_cross 65946 6 mo 14 mo	F1 AD-BXD	55	both	23-57wks	N=6

Neuner1:48	Neuner1	inclined screen test	(none)	mean time to right body when placed face down on 45 degree inclined screen (negative geotaxis), 3 min test, across 3 trials [s] neg_geotaxis 65948 6 mo 14 mo	F1 AD-BXD	55	both	23-57wks	N=6

Neuner1:50	Neuner1	grip strength	(none)	mean grip strength across 3 trials [g] grip_strength 65950 6 mo 14 mo	F1 AD-BXD	55	both	23-57wks	N=6

Neuner1:52	Neuner1	behavior observation	(none)	sensorimotor composite score across narrow beam, inclined screen, grip strength tasks, derived by Z-scores [score] sensorimotor 65952 6 mo 14 mo	F1 AD-BXD	55	both	23-57wks	N=6

Neuner1:54	Neuner1	elevated plus maze	(none)	distance traveled in elevated plus maze, 6 min test [m] epm_dist 65954 6 mo 14 mo	F1 AD-BXD	55	both	23-57wks	N=6

Neuner1:56	Neuner1	elevated plus maze	(none)	number of arm entries in elevated plus maze, 6 min test [n] epm_arm_ent 65956 6 mo 14 mo	F1 AD-BXD	55	both	23-57wks	N=6

Neuner1:58	Neuner1	elevated plus maze	(none)	percentage of entries into open arms in elevated plus maze, 6 min test [%] epm_open_ent 65958 6 mo 14 mo	F1 AD-BXD	55	both	23-57wks	N=6

Neuner1:60	Neuner1	elevated plus maze	(none)	percentage of time spent in open arms of elevated plus maze, 6 min test [%] epm_open_time 65960 6 mo 14 mo	F1 AD-BXD	55	both	23-57wks	N=6

Neuner1:62	Neuner1	fear conditioning test	(none)	percentage of time spent freezing during 40 s interval post shock at 6 mo of age, day 1 contextual fear conditioning [%] cfc_6mo 65962 baseline 1st shock 2nd shock 3rd shock 4th shock	F1 AD-BXD	50	both	23-25wks	N=3

Neuner1:67	Neuner1	fear conditioning test	(none)	percentage of time spent freezing during 40 s interval post shock at 14 mo of age, day 1 contextual fear conditioning [%] cfc_14mo 65967 baseline 1st shock 2nd shock 3rd shock 4th shock	F1 AD-BXD	51	both	55-57wks	N=4

Neuner1:72	Neuner1	fear conditioning test	(none)	change in percentage of time spent freezing from 1st post-shock to 4th post-shock (slope), day 1 contextual fear acquisition cfa_change 65972 6 mo 14 mo	F1 AD-BXD	55	both	23-57wks	N=3

Neuner1:74	Neuner1	fear conditioning test	(none)	percentage of time spent freezing at 6 mo of age, day 2 contextual fear memory, 10 min test [%] cfm_6mo 65974 1st 2 min 2nd 2 min 3rd 2 min 4th 2 min 5th 2 min total	F1 AD-BXD	50	both	23-25wks	N=3

Neuner1:80	Neuner1	fear conditioning test	(none)	percentage of time spent freezing at 14 mo of age, day 2 contextual fear memory, 10 min test [%] cfm_14mo 65980 1st 2 min 2nd 2 min 3rd 2 min 4th 2 min 5th 2 min total	F1 AD-BXD	51	both	55-57wks	N=4

Neuner1: Motor function, spatial learning and memory function, anxiety-related behaviors, and beta-amyloid levels in Alzheimer’s Disease mouse models (AD-BXD) (2019)

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