MPD: Data set: Schughart4

Leist SR, Pilzner C, van den Brand JM, Dengler L, Geffers R, Kuiken T, Balling R, Kollmus H, Schughart K. Influenza H3N2 infection of the collaborative cross founder strains reveals highly divergent host responses and identifies a unique phenotype in CAST/EiJ mice. BMC Genomics. 2016 Feb 27;17:143. doi: 10.1186/s12864-016-2483-y. PubMed 26921172 FullText

Schughart4 downloads
• Download Schughart4 project data set animal data, as uploaded
• Download Schughart4 animal data matrix with factor-related expansion applied as necessary
• Download Schughart4 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Schughart4 measures:

MA:0000059 blood
MA:0000415 lung
MP:0000217 abnormal leukocyte cell number
MP:0000717 abnormal lymphocyte cell number
MP:0001259 abnormal body weight
MP:0012441 abnormal monocyte cell number
MP:0020185 altered susceptibility to viral infection
MP:0020201 abnormal granulocyte number
VT:0000217 leukocyte quantity
VT:0000223 monocyte quantity
VT:0000334 granulocyte quantity
VT:0000717 lymphocyte quantity
VT:0001259 body mass
VT:0010439 response to viral infection trait

Investigators	Sarah R Leist Helmholtz Center for Infection Research, Braunschweig GERMANY Heike Kollmus Helmholtz Center for Infection Research, Braunschweig GERMANY Klaus Schughart Helmholtz Center for Infection Research, Braunschweig GERMANY
Contact	Klaus Schughart Klaus.Schughart@helmholtz-hzi.de Lab web site
Part of a series:	• study 1 see Schughart3 • study 2 (this project) Schughart4 • study 3 see Schughart5
Project type	Phenotype strain survey data set
MPD identifiers	Schughart4 MPD:587
Data changelog	No updates/corrections. Initial release date: 01/2017.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Mice infected with influenza A H3N2 virus (one dose) were analyzed for daily weight loss for up to 30 days. The mouse-adapted virus strain influenza A/HK/01/68 was used. Viral loads and leukocyte profiles are also available. Overall survival and mortality rates are available from females and males in Schughart3, and lung weights are available from females in Schughart5.

Procedures conducted:

• body weight		Influenza A (H3N2) virus vs. controls. Several timepoints.
• complete blood count		White blood cell count and differentials. Influenza A (H3N2) virus vs. controls.
• in vitro assay		Viral load in lungs after infection with influenza A (H3N2) virus.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Schughart4:1	Schughart4	body weight	(none)	body weight pre-infection [g] bw 58701 control group treated group	inbred w/CC8	8	f	8-12wks	N=13

Schughart4:11	Schughart4	body weight	(none)	body weight as percent of baseline, control (saline) group [%] pctbw_ctrl 58711 days post-treatment (saline): 1 2 3	inbred w/CC8	8	f	8-12wks	N=6

Schughart4:21	Schughart4	body weight	influenza A (H3N2) virus	body weight as percent of baseline, infected group [%] pctbw_trt 58721 days post-infection: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 18 30	inbred w/CC8	8	f	8-12wks	N=11

Schughart4:41	Schughart4	complete blood count	influenza A (H3N2) virus	white blood cell count (WBC; per volume x 10³), control group, day 3 [n/µL] WBC_ctrl_d3 58741	inbred w/CC8	8	f	8-12wks	N=5

Schughart4:43	Schughart4	complete blood count	influenza A (H3N2) virus	white blood cell count (WBC; per volume x 10³), treated group [n/µL] WBC_trt 58743 days post-infection: 3 5 8 18 30	inbred w/CC8	8	f	8-12wks	N=6

Schughart4:51	Schughart4	complete blood count	influenza A (H3N2) virus	lymphocyte differential (percent of total WBC), control group, day 3 [%] pctLYM_ctrl_d3 58751	inbred w/CC8	8	f	8-12wks	N=5

Schughart4:53	Schughart4	complete blood count	influenza A (H3N2) virus	lymphocyte differential (LYM; percentage of total WBC), treated group [%] pctLYM_trt 58753 days post-infection: 3 5 8 18 30	inbred w/CC8	8	f	8-12wks	N=6

Schughart4:61	Schughart4	complete blood count	influenza A (H3N2) virus	monocyte differential (percent of total WBC), control group, day 3 [%] pctMONO_ctrl_d3 58761	inbred w/CC8	8	f	8-12wks	N=5

Schughart4:63	Schughart4	complete blood count	influenza A (H3N2) virus	monocyte differential (MONO; percentage of total WBC), treated group [%] pctMONO_trt 58763 days post-infection: 3 5 8 18 30	inbred w/CC8	8	f	8-12wks	N=6

Schughart4:71	Schughart4	complete blood count	influenza A (H3N2) virus	granulocyte differential (percent of total WBC), control group, day 3 [%] pctGRA_ctrl_d3 58771	inbred w/CC8	8	f	8-12wks	N=5

Schughart4:73	Schughart4	complete blood count	influenza A (H3N2) virus	granulocyte differential (GRA; percentage of total WBC), treated group [%] pctGRA_trt 58773 days post-infection: 3 5 8 18 30	inbred w/CC8	8	f	8-12wks	N=6

Schughart4:83	Schughart4	in vitro assay	influenza A (H3N2) virus	viral load in lungs (titer, FFU per lung) log10 (x+1) [log(n)] titer_trt 58783 days post-infection: 3 5 8	inbred w/CC8	8	f	8-12wks	N=5

Schughart4: Susceptibility to influenza A (H3N2) viral infection: body weight, viral loads and leukocyte profiles in females of the 8 inbred founder strains of the Collaborative Cross (2016)