Male BXD mice were fed either a standard chow diet (CD) or a high-fat diet (HFD) from 8 to 29 weeks of age. The effects of diet on the accumulation of 27 different bile acids (BAs) in plasma, feces and liver were assessed by liquid chromatography - high-resolution mass spectrometry (LC-HRMS). The effects of HFD diet were measured for body weight at 8 wks, at 20 wks (at 9 am and immediately after the glucose kinetic test), and prior to sacrifice. Postprandial plasma glucose (following Ensure Plus oral gavage) and plasma BA levels were both determined at 0, 30, 60 min at 20 wks for CD and HFD fed mice. BAs in feces were measured at 24 wks, and in liver at 29 wks. Organs were weighed at sacrifice (29 wks).

Experimental groups in this study:
• Chow diet (CD)     • High fat diet (HFD)    

Procedures conducted:

• body weight		At 8, 20 and 29 wks of age.
• glucose tolerance		Plasma glucose levels at 0, 30, 60 min post oral gavage of Ensure Plus, overday fast.
• biomarker quantification		Bile acid levels in plasma, feces, and liver.
• organ weights		Liver, spleen, kidney, fat pads, muscles, cecum.
• organ dimensions		Small intestine length.

Ontology terms mapped to Schoonjans1 measures:

  MA:0000059   blood
  MA:0000141   spleen
  MA:0000328   intestine
  MA:0000334   cecum
  MA:0000358   liver
  MA:0000368   kidney
  MA:0000473   subcutaneous adipose tissue
  MA:0002306   gastrocnemius
  MA:0002424   soleus
  MA:0002501   plasma
  MA:0002509   feces
  MA:0002550   epididymal fat pad
  MP:0000188   abnormal circulating glucose level MGI
  MP:0001259   abnormal body weight MGI
  MP:0002707   abnormal kidney weight MGI
  MP:0003868   abnormal feces composition MGI
  MP:0004847   abnormal liver weight MGI
  MP:0004951   abnormal spleen weight MGI
  MP:0005291   abnormal glucose tolerance MGI
  MP:0013188   abnormal gastrocnemius weight MGI
  MP:0020165   abnormal soleus weight MGI
  MP:0020997   abnormal subcutaneous adipose tissue amount MGI
  MP:0020998   abnormal epididymal fat pad weight MGI
  MP:0020999   abnormal small intestine length MGI
  MP:0021000   abnormal cecum weight MGI
  VT:0000188   blood glucose amount
  VT:0001259   body mass
  VT:0002707   kidney mass
  VT:0003081   soleus morphology trait
  VT:0003082   gastrocnemius morphology trait
  VT:0003402   liver mass
  VT:0004951   spleen mass
  VT:0010131   cecum mass
  VT:0010195   intestine length
  VT:0010421   epididymal fat pad mass
  VT:0010917   bile acid amount
  VT:0010918   liver bile acid amount
  VT:0010919   feces bile acid amount
  VT:0015088   glucose metabolism trait
  VT:1000472   subcutaneous adipose mass

Investigators	Kristina Schoonjans       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND Hao Li       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND Alessia Perino       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND Qingyao Huang       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND Giacomo von Alvensleben       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND Amir Banaei-Esfahani       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND Johan Auwerx       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Participants	Velazquez-Villegas LA, Gariani K, Ivanisevic J, Korbelius M, Li X, Rapin A, Bou Sleiman M
Contact	Kristina Schoonjans     kristina.schoonjans@epfl.ch     Lab web site
Funding Provided By	École Polytechnique Fédérale de Lausanne; Kristian Gerhard Jebsen Foundation; European Research Council; Swiss National Science Foundation
Project type	Phenotype strain survey data set
MPD identifiers	Schoonjans1     MPD:1049
Data changelog	No updates/corrections.       Initial release date: 09/2022.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.