Schoonjans1: Changes in bile acid pool size and composition in plasma, feces and liver; body and organ weights; and blood glucose levels in male mice of 34 BXD recombinant inbred strains and two parental strains fed chow or high-fat diet (2022)

Li H, Perino A, Huang Q, Von Alvensleben GVG, Banaei-Esfahani A, Velazquez-Villegas LA, Gariani K, Korbelius M, Bou Sleiman M, Imbach J, Sun Y, Li X, Bachmann A, Goeminne LJE, Gallart-Ayala H, Williams EG, Ivanisevic J, Auwerx J, Schoonjans K. Integrative systems analysis identifies genetic and dietary modulators of bile acid homeostasis. Cell Metab. pii: S1550-4131(22)00358-8. doi: 10.1016/j.cmet.2022.08.015.   PubMed 36099916  

Schoonjans1 downloads
• Download Schoonjans1 project data set     animal data, as uploaded
• Download Schoonjans1 animal data matrix     with factor-related expansion applied as necessary
• Download Schoonjans1 strain means, SD, N, etc.     one row per strain/sex/measure
Ontology terms mapped to Schoonjans1 measures:
  MA:0000059   blood
  MA:0000141   spleen
  MA:0000328   intestine
  MA:0000334   cecum
  MA:0000358   liver
  MA:0000368   kidney
  MA:0000473   subcutaneous adipose tissue
  MA:0002306   gastrocnemius
  MA:0002424   soleus
  MA:0002501   plasma
  MA:0002509   feces
  MA:0002550   epididymal fat pad
  MP:0000188   abnormal circulating glucose level
  MP:0001259   abnormal body weight
  MP:0002707   abnormal kidney weight
  MP:0003868   abnormal feces composition
  MP:0004847   abnormal liver weight
  MP:0004951   abnormal spleen weight
  MP:0005291   abnormal glucose tolerance
  MP:0013188   abnormal gastrocnemius weight
  MP:0020165   abnormal soleus weight
  MP:0020997   abnormal subcutaneous adipose tissue amount
  MP:0020998   abnormal epididymal fat pad weight
  MP:0020999   abnormal small intestine length
  MP:0021000   abnormal cecum weight
  VT:0000188   blood glucose amount
  VT:0001259   body mass
  VT:0002707   kidney mass
  VT:0003081   soleus morphology trait
  VT:0003082   gastrocnemius morphology trait
  VT:0003402   liver mass
  VT:0004951   spleen mass
  VT:0010131   cecum mass
  VT:0010195   intestine length
  VT:0010421   epididymal fat pad mass
  VT:0010917   bile acid amount
  VT:0010918   liver bile acid amount
  VT:0010919   feces bile acid amount
  VT:0015088   glucose metabolism trait
  VT:1000472   subcutaneous adipose mass

Investigators Kristina Schoonjans       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Hao Li       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Alessia Perino       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Qingyao Huang       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Giacomo von Alvensleben       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Amir Banaei-Esfahani       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Johan Auwerx       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Participants Velazquez-Villegas LA, Gariani K, Ivanisevic J, Korbelius M, Li X, Rapin A, Bou Sleiman M
ContactKristina Schoonjans     Lab web site
Funding Provided ByÉcole Polytechnique Fédérale de Lausanne; Kristian Gerhard Jebsen Foundation; European Research Council; Swiss National Science Foundation
Project type Phenotype strain survey data set
MPD identifiersSchoonjans1     MPD:1049
Data changelog No updates/corrections.       Initial release date: 09/2022.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
Male BXD mice were fed either a standard chow diet (CD) or a high-fat diet (HFD) from 8 to 29 weeks of age. The effects of diet on the accumulation of 27 different bile acids (BAs) in plasma, feces and liver were assessed by liquid chromatography - high-resolution mass spectrometry (LC-HRMS). The effects of HFD diet were measured for body weight at 8 wks, at 20 wks (at 9 am and immediately after the glucose kinetic test), and prior to sacrifice. Postprandial plasma glucose (following Ensure Plus oral gavage) and plasma BA levels were both determined at 0, 30, 60 min at 20 wks for CD and HFD fed mice. BAs in feces were measured at 24 wks, and in liver at 29 wks. Organs were weighed at sacrifice (29 wks).

Experimental groups in this study:
• Chow diet (CD)     • High fat diet (HFD)    

Procedures conducted:
• body weight  At 8, 20 and 29 wks of age.
• glucose tolerance  Plasma glucose levels at 0, 30, 60 min post oral gavage of Ensure Plus, overday fast.
• biomarker quantification  Bile acid levels in plasma, feces, and liver.
• organ weights  Liver, spleen, kidney, fat pads, muscles, cecum.
• organ dimensions  Small intestine length.

Mice: BXD w/par   36 strains   ♂   age 8-29 wks   2 experimental groups