Project protocol - Contents Workflow and sampling Equipment and supplies Reagents and solutions Procedure: Voluntary exercise Data Workflow and sampling Step Procedure Equipment Data collected 1 Voluntary wheel running Standard laboratory cages with attached running wheels Distance traveled, duration, average speed 2 Measure food intake and assess body composition Scales and MRI See McMullan3 Equipment and supplies Running wheels (1.1 m circumference) (Lafayette Industries, Lafayette IN) Automated Activity Wheel Monitoring Program (AWM, Lafayette Industries, Lafayette IN) Procedure: Voluntary exercise Mice are individually housed in standard laboratory cages with attached running wheels. Mice are allowed ad libitum access to running wheels for a month (28 days). Voluntary wheel running is recorded continuously in 1-min intervals (as number of revolutions) using software from the manufacturer. Data collected by investigator Daily distance traveled (total revolutions x 1.1 m) Daily duration (cumulative 1-min intervals in which at least 1 revolution is recorded) Daily average speed (total revolutions/total duration)
Step Procedure Equipment Data collected 1 Voluntary wheel running Standard laboratory cages with attached running wheels Distance traveled, duration, average speed 2 Measure food intake and assess body composition Scales and MRI See McMullan3