MPD: Data set: JaxKOMP-EAP

JaxKOMP-EAP: Multisystem phenotyping of KOMP strains and C57BL/6NJ controls, early adult mice (2021)

JAX KOMP Phenotyping Center Stephen A Murray, Jacqui White, Robert E Braun With:

The JaxKOMP Early Adult Phenotyping project is an ongoing phenotyping pipeline currently with over 1700 knockout strains/genotypes using IMPC JaxKOMP protocols. Mice are tested starting at 8 wks of age and ending at 18 wks. Additional knockout lines continue to be run through the pipeline and data will be updated here quarterly. The primary unit of analysis for MPD purposes is "effect size" which is computed from the raw data using PhenStat algorithms, indicating the difference between a knockout genotype vs. C57BL/6NJ controls.

Procedures conducted:

• grip strength		3 trials.
• open field test		Locomotor, exploratory, and anxiety-related behavior. 20 min test, some at 5 min intervals.
• observation (SHIRPA)		Behavior, and neurosensory responses (SHIRPA protocol).
• Y-maze		Spontaneous alternation and other parameters. 8 min test.
• hole board test		Exploratory behavior.
• light-dark box		Anxiety, avoidance behavior. 20 min test.
• ABR test		Auditory brainstem response.
• acoustic startle test		Acoustic startle response, prepulse inhibition.
• rotarod		Latency to fall from accelerating rotarod. 5 trials.
• ECG		Heart rate and wave intervals. Conscious.
• tail suspension test		Latency to immobility, duration of immobility.
• glucose tolerance		0 to 120 min after 20% glucose i.p., 16h fast.
• urinalysis		Microalbumin, creatinine, ratio.
• lipid profile		Cholesterol, HDL, triglycerides, fatty acids. 4h fast, plasma.
• X-ray		Counts of vertebrae, ribs, digits, and other categorical parameters.
• DXA		Body mass, fat mass, lean mass, bone mineral density and content, bone area.
• ophthalmoscopy		Eye morphology.
• slit lamp		Eye morphology.
• monitoring system		Sleep parameters, light and dark periods.
• electroretinography		Photoreceptor measures.
• metabolic panel		Clinical blood chemistry (plasma). No fast.
• electroconvulsion test		Stimulus levels.
• organ weights		Heart weight. Raw and normalized to body weight.
• complete blood count		Hematology parameters.
• hormone quantification		Insulin (plasma).

JaxKOMP-EAP downloads
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Ontology terms mapped to JaxKOMP-EAP measures:

Institutional authorship	JAX KOMP Phenotyping Center
Investigators	Stephen A Murray The Jackson Laboratory, Bar Harbor, ME Jacqui White The Jackson Laboratory, Bar Harbor, ME Robert E Braun The Jackson Laboratory, Bar Harbor, ME
Participants
Contact	Stephen A Murray Steve.Murray@jax.org
Part of a series:	• Early Adult (this project) JaxKOMP-EAP • Late Adult see JaxKOMP-LAP
Funding Provided By	Office of The Director, National Institutes of Health under Award Number UM1OD023222; The Jackson Laboratory Director's Innovation Fund
Project type	KOMP study
MPD identifiers	JaxKOMP-EAP MPD:516
Data changelog	2 updates/corrections. Initial release date: 09/2020.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Phenotypes N=389

Genotypes N=2199 ♀ ♂

QC views (mpdpreview.jax.org) include data collected thru 2023-06-23.

Data collection is ongoing and updates are posted here periodically.
Effect size values are computed for eligible genotypes that have finished the phenotyping pipeline.