JaxKOMP-EAP: Multisystem phenotyping of KOMP strains and C57BL/6NJ controls, early adult mice (2020)

Jackson Laboratory KOMP phenotyping group   Stephen A Murray, Jacqui White, Robert E Braun

         
Ontology terms mapped to JaxKOMP-EAP measures:
    MA:0000004   trunk
    MA:0000008   tail
    MA:0000009   adipose tissue
    MA:0000023   head
    MA:0000025   forelimb
    MA:0000026   hindlimb
    MA:0000030   pelvis
    MA:0000059   blood
    MA:0000072   heart
    MA:0000141   spleen
    MA:0000151   skin
    MA:0000155   coat hair
    MA:0000163   vibrissa
    MA:0000167   central nervous system
    MA:0000236   ear
    MA:0000261   eye
    MA:0000262   eye anterior chamber
    MA:0000266   cornea
    MA:0000268   eyelid
    MA:0000273   iris
    MA:0000275   lens
    MA:0000276   retina
    MA:0000278   optic disc
    MA:0000309   vertebra
    MA:0000310   caudal vertebra
    MA:0000311   cervical vertebra
    MA:0000312   lumbar vertebra
    MA:0000314   thoracic vertebra
    MA:0000315   rib
    MA:0000316   cranium
    MA:0000319   joint
    MA:0000348   tooth
    MA:0000615   forelimb muscle
    MA:0000690   limb digit
    MA:0000692   limb muscle
    MA:0001283   hyaloid vascular plexus
    MA:0001292   pupil
    MA:0001329   clavicle
    MA:0001330   scapula
    MA:0001356   humerus
    MA:0001357   radius
    MA:0001358   ulna
    MA:0001359   femur
    MA:0001360   fibula
    MA:0001361   tibia
    MA:0001420   coccygeal vertebra
    MA:0001459   bone
    MA:0001487   mandible
    MA:0001491   maxilla
    MA:0001497   zygomatic bone
    MA:0001752   reproductive organ
    MA:0001910   snout
    MA:0002443   auditory system
    MA:0002462   vitreous body
    MA:0002474   mouth
    MA:0002501   plasma
    MA:0002545   urine
    MA:0002703   nail
    MA:0002714   autopod
    MA:0003002   tissue
    MA:0003010   footpad
    MP:0000137   abnormal vertebrae morphology
    MP:0000149   abnormal scapula morphology
    MP:0000150   abnormal rib morphology
    MP:0000160   kyphosis
    MP:0000162   lordosis
    MP:0000180   abnormal circulating cholesterol level
    MP:0000184   abnormal circulating HDL cholesterol level
    MP:0000188   abnormal circulating glucose level
    MP:0000199   abnormal circulating serum albumin level
    MP:0000202   abnormal circulating alkaline phosphatase level
    MP:0000203   abnormal circulating aspartate transaminase level
    MP:0000217   abnormal leukocyte cell number
    MP:0000226   abnormal mean corpuscular volume
    MP:0000367   abnormal coat/ hair morphology
    MP:0000432   abnormal head morphology
    MP:0000438   abnormal cranium morphology
    MP:0000443   abnormal snout morphology
    MP:0000452   abnormal mouth morphology
    MP:0000550   abnormal forelimb morphology
    MP:0000552   abnormal radius morphology
    MP:0000556   abnormal hindlimb morphology
    MP:0000558   abnormal tibia morphology
    MP:0000572   abnormal autopod morphology
    MP:0000579   abnormal nail morphology
    MP:0000717   abnormal lymphocyte cell number
    MP:0001005   abnormal retinal rod cell morphology
    MP:0001006   abnormal retinal cone cell morphology
    MP:0001256   abnormal body length
    MP:0001259   abnormal body weight
    MP:0001289   persistence of hyaloid vascular system
    MP:0001303   abnormal lens morphology
    MP:0001312   abnormal cornea morphology
    MP:0001317   abnormal pupil morphology
    MP:0001325   abnormal retina morphology
    MP:0001340   abnormal eyelid morphology
    MP:0001362   abnormal anxiety-related response
    MP:0001392   abnormal locomotor behavior
    MP:0001406   abnormal gait
    MP:0001408   stereotypic behavior
    MP:0001486   abnormal startle reflex
    MP:0001513   limb grasping
    MP:0001515   abnormal grip strength
    MP:0001516   abnormal motor coordination/balance
    MP:0001529   abnormal vocalization
    MP:0001553   abnormal circulating free fatty acids level
    MP:0001560   abnormal circulating insulin level
    MP:0001562   abnormal circulating calcium level
    MP:0001565   abnormal circulating phosphate level
    MP:0001569   abnormal circulating bilirubin level
    MP:0001573   abnormal circulating alanine transaminase level
    MP:0001586   abnormal erythrocyte cell number
    MP:0001588   abnormal hemoglobin
    MP:0001589   abnormal mean corpuscular hemoglobin
    MP:0001629   abnormal heart rate
    MP:0001650   abnormal seizure response to electrical stimulation
    MP:0001758   abnormal urine glucose level
    MP:0001776   abnormal circulating sodium level
    MP:0002060   abnormal skin morphology
    MP:0002061   abnormal aggression-related behavior
    MP:0002074   abnormal hair texture
    MP:0002075   abnormal coat/hair pigmentation
    MP:0002089   abnormal postnatal growth/weight/body size
    MP:0002092   abnormal eye morphology
    MP:0002095   abnormal skin pigmentation
    MP:0002098   abnormal vibrissa morphology
    MP:0002100   abnormal tooth morphology
    MP:0002102   abnormal ear morphology
    MP:0002110   abnormal digit morphology
    MP:0002111   abnormal tail morphology
    MP:0002160   abnormal reproductive system morphology
    MP:0002544   brachydactyly
    MP:0002586   abnormal platelet volume
    MP:0002596   abnormal hematocrit
    MP:0002602   abnormal eosinophil cell number
    MP:0002668   abnormal circulating potassium level
    MP:0002699   abnormal vitreous body morphology
    MP:0002862   altered righting response
    MP:0003018   abnormal circulating chloride level
    MP:0003088   abnormal prepulse inhibition
    MP:0003107   abnormal response to novelty
    MP:0003360   abnormal depression-related behavior
    MP:0003492   abnormal involuntary movement
    MP:0003671   abnormal eyelid aperture
    MP:0003795   abnormal bone structure
    MP:0003860   abnormal carbon dioxide level
    MP:0003897   abnormal ST segment
    MP:0003898   abnormal QRS complex
    MP:0003899   abnormal QT interval
    MP:0003901   abnormal PR interval
    MP:0003956   abnormal body size
    MP:0003959   abnormal lean body mass
    MP:0004022   abnormal cone electrophysiology
    MP:0004152   abnormal circulating iron level
    MP:0004174   abnormal spine curvature
    MP:0004222   iris synechia
    MP:0004703   abnormal vertebral column morphology
    MP:0004738   abnormal auditory brainstem response
    MP:0004857   abnormal heart weight
    MP:0004951   abnormal spleen weight
    MP:0005102   abnormal iris pigmentation
    MP:0005108   abnormal ulna morphology
    MP:0005205   abnormal eye anterior chamber morphology
    MP:0005265   abnormal blood urea nitrogen level
    MP:0005270   abnormal zygomatic bone morphology
    MP:0005287   narrow eye opening
    MP:0005310   abnormal salivary gland physiology
    MP:0005316   abnormal response to tactile stimuli
    MP:0005328   abnormal circulating creatinine level
    MP:0005451   abnormal body composition
    MP:0005452   abnormal adipose tissue amount
    MP:0005457   abnormal percent body fat/body weight
    MP:0005551   abnormal eye electrophysiology
    MP:0005640   abnormal mean corpuscular hemoglobin concentration
    MP:0006315   abnormal urine protein level
    MP:0008259   abnormal optic disk morphology
    MP:0008849   abnormal hemoglobin concentration distribution width
    MP:0010066   abnormal red blood cell distribution width
    MP:0010122   abnormal bone mineral content
    MP:0010504   abnormal RR interval
    MP:0011396   abnormal sleep behavior
    MP:0011434   abnormal urine magnesium level
    MP:0011469   abnormal urine creatinine level
    MP:0011496   abnormal head size
    MP:0011888   abnormal circulating total protein level
    MP:0011898   abnormal platelet cell number
    MP:0011913   abnormal reticulocyte cell number
    MP:0011918   abnormal PQ interval
    MP:0011919   abnormal R wave
    MP:0011969   abnormal circulating triglyceride level
    MP:0012122   abnormal iris transillumination
    MP:0012321   abnormal total tissue mass
    MP:0012360   abnormal large unstained cell number
    MP:0012397   abnormal nucleated erythrocyte cell number
    MP:0012440   abnormal neutrophil cell number
    MP:0012441   abnormal monocyte cell number
    MP:0012442   abnormal basophil cell number
    MP:0013176   abnormal tail position or orientation
    MP:0013189   abnormal exploration in a new environment
    MP:0020147   abnormal defecation amount
    MP:0020153   abnormal urine albumin level
    MP:0020218   abnormal tear production
    VT:0000002   head size trait
    VT:0000003   adipose morphology trait
    VT:0000004   forelimb conformation trait
    VT:0000016   hindlimb conformation trait
    VT:0000137   vertebra morphology trait
    VT:0000149   scapula morphology trait
    VT:0000150   rib morphology trait
    VT:0000180   blood cholesterol amount
    VT:0000184   blood HDL cholesterol amount
    VT:0000188   blood glucose amount
    VT:0000199   blood albumin amount
    VT:0000202   blood alkaline phosphatase amount
    VT:0000203   blood aspartate transaminase amount
    VT:0000217   leukocyte quantity
    VT:0000222   neutrophil quantity
    VT:0000223   monocyte quantity
    VT:0000231   heart excitatory physiology trait
    VT:0000248   erythrocyte size trait
    VT:0000366   brainstem auditory evoked potential
    VT:0000432   head morphology trait
    VT:0000438   skull morphology trait
    VT:0000443   snout morphology trait
    VT:0000452   mouth morphology trait
    VT:0000550   forelimb morphology trait
    VT:0000552   radius morphology trait
    VT:0000556   hindlimb morphology trait
    VT:0000558   tibia morphology trait
    VT:0000579   nail morphology trait
    VT:0000588   tail diameter
    VT:0000623   salivation trait
    VT:0000717   lymphocyte quantity
    VT:0001005   retinal rod cell morphology trait
    VT:0001006   retinal cone cell morphology trait
    VT:0001256   body length
    VT:0001259   body mass
    VT:0001289   hyaloid capillary system development trait
    VT:0001303   lens morphology trait
    VT:0001312   cornea morphology trait
    VT:0001317   pupil morphology trait
    VT:0001322   iris morphology trait
    VT:0001325   retina morphology trait
    VT:0001340   eyelid morphology trait
    VT:0001349   tear production trait
    VT:0001392   locomotor behavior trait
    VT:0001501   sleep behavior trait
    VT:0001516   motor coordination/balance trait
    VT:0001529   vocalization trait
    VT:0001553   blood free fatty acid amount
    VT:0001560   blood insulin amount
    VT:0001562   blood calcium amount
    VT:0001565   blood phosphate amount
    VT:0001569   blood bilirubin amount
    VT:0001573   blood alanine transaminase amount
    VT:0001586   erythrocyte quantity
    VT:0001588   blood hemoglobin amount
    VT:0001756   urine production trait
    VT:0001757   urine molecular composition trait
    VT:0001758   urine glucose amount
    VT:0001776   blood sodium amount
    VT:0002092   eye morphology trait
    VT:0002095   skin pigmentation trait
    VT:0002096   skin morphology trait
    VT:0002098   vibrissa morphology trait
    VT:0002100   tooth morphology trait
    VT:0002102   ear morphology trait
    VT:0002110   digit morphology trait
    VT:0002111   tail morphology trait
    VT:0002136   kidney physiology trait
    VT:0002160   reproductive system morphology trait
    VT:0002329   blood gas amount
    VT:0002447   erythrocyte morphology trait
    VT:0002602   eosinophil quantity
    VT:0002607   basophil quantity
    VT:0002644   blood triglyceride amount
    VT:0002668   blood potassium amount
    VT:0002699   vitreous body morphology trait
    VT:0002758   tail length
    VT:0002792   retina blood vessel morphology trait
    VT:0002847   glomerular filtration trait
    VT:0002871   urine albumin amount
    VT:0003018   blood chloride amount
    VT:0003051   tail shape trait
    VT:0003088   prepulse inhibition trait
    VT:0003135   reticulocyte quantity
    VT:0003179   platelet quantity
    VT:0003492   involuntary movement trait
    VT:0003671   eyelid aperture
    VT:0003800   digit quantity
    VT:0004152   blood iron amount
    VT:0004262   physical strength trait
    VT:0004271   paw/hand/foot/hoof morphology trait
    VT:0004951   spleen mass
    VT:0005007   bone mineral mass
    VT:0005102   iris pigmentation trait
    VT:0005108   ulna morphology trait
    VT:0005205   eye anterior chamber morphology trait
    VT:0005265   blood urea nitrogen amount
    VT:0005270   zygomatic bone morphology trait
    VT:0005316   tactition trait
    VT:0005328   blood creatinine amount
    VT:0005452   adipose amount
    VT:0005567   blood total protein amount
    VT:0006215   optic disc morphology trait
    VT:0006276   autonomic nervous system physiology trait
    VT:0007028   heart mass
    VT:0010003   bone morphology trait
    VT:0010080   skeletal muscle physiology trait
    VT:0010086   vertebral column morphology trait
    VT:0010457   platelet size trait
    VT:0010459   behavioral response to novel environment trait
    VT:0010462   defecation behavior trait
    VT:0010471   exploratory behavior trait
    VT:0010482   body fat mass
    VT:0010483   body lean mass
    VT:0010716   anxiety-related behavior trait
    VT:0010725   startle reflex trait
    VT:0010731   gait trait
    VT:0010748   stereotypic behavior trait
    VT:0015014   aggression-related behavior trait
    VT:0015015   depression-related behavior trait
    VT:0015058   brain physiology trait
    VT:0100005   body size trait
    VT:1000000   body conformation trait
    VT:1000775   integumentary system trait
    VT:2000009   heart pumping trait
Institutional authorship Jackson Laboratory KOMP phenotyping group
Investigators Stephen A Murray       The Jackson Laboratory,  Bar Harbor, ME
Jacqui White       The Jackson Laboratory,  Bar Harbor, ME
Robert E Braun       The Jackson Laboratory,  Bar Harbor, ME
ContactStephen A Murray     Steve.Murray@jax.org
Part of a series: • Early Adult   (this project) JaxKOMP-EAP
• Late Adult   see JaxKOMP-LAP
Funding Provided ByOffice of The Director, National Institutes of Health under Award Number UM1OD023222; The Jackson Laboratory Director's Innovation Fund
Project type KOMP study
MPD identifiersJaxKOMP-EAP     MPD:516
Data changelog No updates/corrections.       Initial release date: 09/2020.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
The JaxKOMP Early Adult Phenotyping project is an ongoing phenotyping pipeline currently with over 1700 knockout strains/genotypes using IMPC JaxKOMP protocols. Mice are tested starting at 8 wks of age and ending at 18 wks. Additional knockout lines continue to be run through the pipeline and data will be updated here quarterly. The primary unit of analysis for MPD purposes is "effect size" which is computed from the raw data using PhenStat algorithms, indicating the difference between a knockout genotype vs. C57BL/6NJ controls.

Procedures conducted:
• grip strength  3 trials.
• open field test  Locomotor, exploratory, and anxiety-related behavior. 20 min test, some at 5 min intervals.
• observation (SHIRPA)  Behavior, and neurosensory responses (SHIRPA protocol).
• Y-maze  Spontaneous alternation and other parameters. 8 min test.
• hole board test  Exploratory behavior.
• light-dark box  Anxiety, avoidance behavior. 20 min test.
• ABR test  Auditory brainstem response.
• acoustic startle test  Acoustic startle response, prepulse inhibition.
• rotarod  Latency to fall from accelerating rotarod. 5 trials.
• ECG  Heart rate and wave intervals. Conscious.
• tail suspension test  Latency to immobility, duration of immobility.
• glucose tolerance  0 to 120 min after 20% glucose i.p., 16h fast.
• urinalysis  Microalbumin, creatinine, ratio.
• lipid profile  Cholesterol, HDL, triglycerides, fatty acids. 4h fast, plasma.
• X-ray  Counts of vertebrae, ribs, digits, and other categorical parameters.
• DXA  Body mass, fat mass, lean mass, bone mineral density and content, bone area.
• ophthalmoscopy  Eye morphology.
• slit lamp  Eye morphology.
• monitoring system  Sleep parameters, light and dark periods.
• electroretinography  Photoreceptor measures.
• metabolic panel  Clinical blood chemistry (plasma). No fast.
• electroconvulsion test  Stimulus levels.
• organ weights  Heart weight. Raw and normalized to body weight.
• complete blood count  Hematology parameters.
• hormone quantification  Insulin (plasma).

Phenotypes     N=364

Genotypes   N=1820   ♀ ♂


These data are current to 2020-06-26 ... and are updated quarterly.