JaxKOMP-LAP: Multisystem phenotyping of KOMP strains and C57BL/6NJ controls, late adult mice (2021)

JAX KOMP Phenotyping Center   Stephen A Murray, Jacqui White, Robert E Braun   With:

         
Ontology terms mapped to JaxKOMP-LAP measures:
  MA:0000004   trunk
  MA:0000008   tail
  MA:0000009   adipose tissue
  MA:0000023   head
  MA:0000025   forelimb
  MA:0000026   hindlimb
  MA:0000059   blood
  MA:0000072   heart
  MA:0000151   skin
  MA:0000155   coat hair
  MA:0000163   vibrissa
  MA:0000167   central nervous system
  MA:0000236   ear
  MA:0000261   eye
  MA:0000262   eye anterior chamber
  MA:0000266   cornea
  MA:0000268   eyelid
  MA:0000273   iris
  MA:0000275   lens
  MA:0000276   retina
  MA:0000278   optic disc
  MA:0000348   tooth
  MA:0000615   forelimb muscle
  MA:0000690   limb digit
  MA:0000692   limb muscle
  MA:0001283   hyaloid vascular plexus
  MA:0001292   pupil
  MA:0001459   bone
  MA:0001752   reproductive organ
  MA:0001910   snout
  MA:0002443   auditory system
  MA:0002462   vitreous body
  MA:0002474   mouth
  MA:0002501   plasma
  MA:0002703   nail
  MA:0002714   autopod
  MA:0003002   tissue
  MA:0003010   footpad
  MP:0000180   abnormal circulating cholesterol level
  MP:0000184   abnormal circulating HDL cholesterol level
  MP:0000188   abnormal circulating glucose level
  MP:0000199   abnormal circulating serum albumin level
  MP:0000202   abnormal circulating alkaline phosphatase level
  MP:0000203   abnormal circulating aspartate transaminase level
  MP:0000217   abnormal leukocyte cell number
  MP:0000226   abnormal mean corpuscular volume
  MP:0000367   abnormal coat/ hair morphology
  MP:0000432   abnormal head morphology
  MP:0000443   abnormal snout morphology
  MP:0000452   abnormal mouth morphology
  MP:0000550   abnormal forelimb morphology
  MP:0000556   abnormal hindlimb morphology
  MP:0000572   abnormal autopod morphology
  MP:0000579   abnormal nail morphology
  MP:0000717   abnormal lymphocyte cell number
  MP:0001256   abnormal body length
  MP:0001259   abnormal body weight
  MP:0001289   persistence of hyaloid vascular system
  MP:0001303   abnormal lens morphology
  MP:0001312   abnormal cornea morphology
  MP:0001317   abnormal pupil morphology
  MP:0001325   abnormal retina morphology
  MP:0001340   abnormal eyelid morphology
  MP:0001362   abnormal anxiety-related response
  MP:0001392   abnormal locomotor behavior
  MP:0001406   abnormal gait
  MP:0001408   stereotypic behavior
  MP:0001486   abnormal startle reflex
  MP:0001513   limb grasping
  MP:0001515   abnormal grip strength
  MP:0001529   abnormal vocalization
  MP:0001553   abnormal circulating free fatty acids level
  MP:0001562   abnormal circulating calcium level
  MP:0001565   abnormal circulating phosphate level
  MP:0001569   abnormal circulating bilirubin level
  MP:0001573   abnormal circulating alanine transaminase level
  MP:0001586   abnormal erythrocyte cell number
  MP:0001588   abnormal hemoglobin
  MP:0001589   abnormal mean corpuscular hemoglobin
  MP:0001629   abnormal heart rate
  MP:0001776   abnormal circulating sodium level
  MP:0002060   abnormal skin morphology
  MP:0002061   abnormal aggression-related behavior
  MP:0002074   abnormal hair texture
  MP:0002075   abnormal coat/hair pigmentation
  MP:0002089   abnormal postnatal growth/weight/body size
  MP:0002092   abnormal eye morphology
  MP:0002095   abnormal skin pigmentation
  MP:0002098   abnormal vibrissa morphology
  MP:0002102   abnormal ear morphology
  MP:0002110   abnormal digit morphology
  MP:0002111   abnormal tail morphology
  MP:0002160   abnormal reproductive system morphology
  MP:0002586   abnormal platelet volume
  MP:0002596   abnormal hematocrit
  MP:0002602   abnormal eosinophil cell number
  MP:0002668   abnormal circulating potassium level
  MP:0002699   abnormal vitreous body morphology
  MP:0002862   altered righting response
  MP:0003018   abnormal circulating chloride level
  MP:0003088   abnormal prepulse inhibition
  MP:0003107   abnormal response to novelty
  MP:0003492   abnormal involuntary movement
  MP:0003671   abnormal eyelid aperture
  MP:0003795   abnormal bone structure
  MP:0003860   abnormal carbon dioxide level
  MP:0003897   abnormal ST segment
  MP:0003898   abnormal QRS complex
  MP:0003899   abnormal QT interval
  MP:0003901   abnormal PR interval
  MP:0003956   abnormal body size
  MP:0003959   abnormal lean body mass
  MP:0004152   abnormal circulating iron level
  MP:0004222   iris synechia
  MP:0004738   abnormal auditory brainstem response
  MP:0004857   abnormal heart weight
  MP:0005102   abnormal iris pigmentation
  MP:0005205   abnormal eye anterior chamber morphology
  MP:0005265   abnormal blood urea nitrogen level
  MP:0005287   narrow eye opening
  MP:0005291   abnormal glucose tolerance
  MP:0005310   abnormal salivary gland physiology
  MP:0005316   abnormal response to tactile stimuli
  MP:0005328   abnormal circulating creatinine level
  MP:0005451   abnormal body composition
  MP:0005452   abnormal adipose tissue amount
  MP:0005457   abnormal percent body fat/body weight
  MP:0005640   abnormal mean corpuscular hemoglobin concentration
  MP:0008259   abnormal optic disk morphology
  MP:0008849   abnormal hemoglobin concentration distribution width
  MP:0010066   abnormal red blood cell distribution width
  MP:0010122   abnormal bone mineral content
  MP:0010504   abnormal RR interval
  MP:0011496   abnormal head size
  MP:0011888   abnormal circulating total protein level
  MP:0011898   abnormal platelet cell number
  MP:0011913   abnormal reticulocyte cell number
  MP:0011918   abnormal PQ interval
  MP:0011919   abnormal R wave
  MP:0011969   abnormal circulating triglyceride level
  MP:0012122   abnormal iris transillumination
  MP:0012321   abnormal total tissue mass
  MP:0012360   abnormal large unstained cell number
  MP:0012397   abnormal nucleated erythrocyte cell number
  MP:0012440   abnormal neutrophil cell number
  MP:0012441   abnormal monocyte cell number
  MP:0012442   abnormal basophil cell number
  MP:0013176   abnormal tail position or orientation
  MP:0013189   abnormal exploration in a new environment
  MP:0020147   abnormal defecation amount
  MP:0020218   abnormal tear production
  VT:0000002   head size trait
  VT:0000003   adipose morphology trait
  VT:0000004   forelimb conformation trait
  VT:0000016   hindlimb conformation trait
  VT:0000180   blood cholesterol amount
  VT:0000184   blood HDL cholesterol amount
  VT:0000188   blood glucose amount
  VT:0000199   blood albumin amount
  VT:0000202   blood alkaline phosphatase amount
  VT:0000203   blood aspartate transaminase amount
  VT:0000217   leukocyte quantity
  VT:0000222   neutrophil quantity
  VT:0000223   monocyte quantity
  VT:0000231   heart excitatory physiology trait
  VT:0000248   erythrocyte size trait
  VT:0000366   brainstem auditory evoked potential
  VT:0000432   head morphology trait
  VT:0000443   snout morphology trait
  VT:0000452   mouth morphology trait
  VT:0000550   forelimb morphology trait
  VT:0000556   hindlimb morphology trait
  VT:0000579   nail morphology trait
  VT:0000588   tail diameter
  VT:0000623   salivation trait
  VT:0000717   lymphocyte quantity
  VT:0001256   body length
  VT:0001259   body mass
  VT:0001289   hyaloid capillary system development trait
  VT:0001303   lens morphology trait
  VT:0001312   cornea morphology trait
  VT:0001317   pupil morphology trait
  VT:0001322   iris morphology trait
  VT:0001325   retina morphology trait
  VT:0001340   eyelid morphology trait
  VT:0001349   tear production trait
  VT:0001392   locomotor behavior trait
  VT:0001529   vocalization trait
  VT:0001553   blood free fatty acid amount
  VT:0001562   blood calcium amount
  VT:0001565   blood phosphate amount
  VT:0001569   blood bilirubin amount
  VT:0001573   blood alanine transaminase amount
  VT:0001586   erythrocyte quantity
  VT:0001588   blood hemoglobin amount
  VT:0001776   blood sodium amount
  VT:0001968   tactile sensory behavior trait
  VT:0002092   eye morphology trait
  VT:0002095   skin pigmentation trait
  VT:0002096   skin morphology trait
  VT:0002098   vibrissa morphology trait
  VT:0002102   ear morphology trait
  VT:0002110   digit morphology trait
  VT:0002111   tail morphology trait
  VT:0002160   reproductive system morphology trait
  VT:0002329   blood gas amount
  VT:0002447   erythrocyte morphology trait
  VT:0002602   eosinophil quantity
  VT:0002607   basophil quantity
  VT:0002644   blood triglyceride amount
  VT:0002668   blood potassium amount
  VT:0002699   vitreous body morphology trait
  VT:0002758   tail length
  VT:0002792   retina blood vessel morphology trait
  VT:0003018   blood chloride amount
  VT:0003051   tail shape trait
  VT:0003088   prepulse inhibition trait
  VT:0003135   reticulocyte quantity
  VT:0003179   platelet quantity
  VT:0003492   involuntary movement trait
  VT:0003671   eyelid aperture
  VT:0003800   digit quantity
  VT:0004152   blood iron amount
  VT:0004262   physical strength trait
  VT:0004271   paw/hand/foot/hoof morphology trait
  VT:0005007   bone mineral mass
  VT:0005102   iris pigmentation trait
  VT:0005205   eye anterior chamber morphology trait
  VT:0005265   blood urea nitrogen amount
  VT:0005328   blood creatinine amount
  VT:0005452   adipose amount
  VT:0005567   blood total protein amount
  VT:0006215   optic disc morphology trait
  VT:0006276   autonomic nervous system physiology trait
  VT:0007028   heart mass
  VT:0010003   bone morphology trait
  VT:0010080   skeletal muscle physiology trait
  VT:0010457   platelet size trait
  VT:0010459   behavioral response to novel environment trait
  VT:0010462   defecation behavior trait
  VT:0010471   exploratory behavior trait
  VT:0010482   body fat mass
  VT:0010483   body lean mass
  VT:0010716   anxiety-related behavior trait
  VT:0010725   startle reflex trait
  VT:0010731   gait trait
  VT:0010748   stereotypic behavior trait
  VT:0015014   aggression-related behavior trait
  VT:0015088   glucose metabolism trait
  VT:0100005   body size trait
  VT:1000000   body conformation trait
  VT:1000775   integumentary system trait
  VT:2000009   heart pumping trait

Institutional authorship JAX KOMP Phenotyping Center
Investigators Stephen A Murray       The Jackson Laboratory,  Bar Harbor, ME
Jacqui White       The Jackson Laboratory,  Bar Harbor, ME
Robert E Braun       The Jackson Laboratory,  Bar Harbor, ME
Participants
ContactStephen A Murray     Steve.Murray@jax.org
Part of a series: • Early Adult   see JaxKOMP-EAP
• Late Adult   (this project) JaxKOMP-LAP
Funding Provided ByOffice of The Director, National Institutes of Health under Award Number UM1OD023222; The Jackson Laboratory Director's Innovation Fund
Project type KOMP study
MPD identifiersJaxKOMP-LAP     MPD:517
Data changelog 2 updates/corrections.       Initial release date: 09/2020.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
The JaxKOMP Late Adult Phenotyping project is an ongoing phenotyping pipeline currently with over 170 knockout strains/genotypes using IMPC JaxKOMP protocols. Mice are tested starting at 65 wks of age and ending at 80 wks. Additional knockout lines continue to be run through the pipeline and data will be updated here quarterly. The primary unit of analysis for MPD purposes is "effect size" which is computed from the raw data using PhenStat algorithms, indicating the difference between a knockout genotype vs. similarly aged C57BL/6NJ controls.

Procedures conducted:
• grip strength  3 trials.
• open field test  Locomotor, exploratory, and anxiety-related behavior. 20 min test, some at 5 min intervals.
• observation (SHIRPA)  Behavior, and neurosensory responses (SHIRPA protocol).
• Y-maze  Spontaneous alternation and other parameters. 8 min test.
• hole board test  Exploratory behavior.
• light-dark box  Anxiety, avoidance behavior. 20 min test.
• ABR test  Auditory brainstem response.
• acoustic startle test  Acoustic startle response, prepulse inhibition.
• ECG  Heart rate and wave intervals. Conscious.
• glucose tolerance  0 to 120 min after 20% glucose i.p., 16h fast.
• DXA  Body mass, fat mass, lean mass, bone mineral density and content, bone area.
• ophthalmoscopy  Eye morphology.
• slit lamp  Eye morphology.
• metabolic panel  Clinical blood chemistry (plasma). No fast.
• organ weights  Heart weight. Raw and normalized to body weight.
• complete blood count  Hematology parameters.

Phenotypes     N=281

Genotypes   N=164   ♀ ♂


QC views (mpdpreview.jax.org) include data collected thru 2021-06-14.

Data collection is ongoing and updates are posted here periodically.
Effect size values are computed for eligible genotypes that have finished the phenotyping pipeline.