Albers1 project protocol

Diet-induced changes in plasma phospholipid transfer protein activity, lipids, and lipoproteins in 15 inbred strains of mice   (1999)

Albers JJ, Paigen B
With: Pitman W, Wolfbauer G, Cheung MC Kennedy H, Tu AY, Marcovina SM

See Albers et al., 1999 for detailed description.

Mice were fed normal chow until 6-8 weeks of age when a high-fat diet was administered (duration 6 weeks).

High Fat Diet Specifications (for details see Nashina et al. 1993):
(w/w) 15% dairy fat, 50% sucrose, 20% casein, 0.5% cholic acid, 1.0% cholesterol, cellulose, vitamins, and minerals

Lipid and phospholipid transfer protein activity (PLTP) analyses:
Mice were fasted 4 h prior to blood collection (retro-orbital bleeding). Samples from 8 mice of each strain (by sex) were pooled. Lipid assessments were carried out in duplicate using previously described methods (Albers et al., 1996). PLTP-specific phospholipid transfer activity was assessed by the transfer of [14C] phosphatidyl-choline from radiolabeled phospholipid liposomes to a preparation of high density lipoprotein (HDL3).