Acclimation and testing periods
Vendor Mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, ME) Ages 6, 12, 20 months Feed All mice were fed Lab diet® 5K52 (6% fat, PMI Nutritional International, Bentwood, MO) ad libitum Water Drinking water was acidified to pH 2.8 to 3.2 to prevent bacterial growth Housing Same-sex mice were housed four per pen in duplex polycarbonate cages (31x31x214cm) equipped with pressurized individually ventilated mouse racks (Thoren Caging Systems Inc.) with HEPA filtered supply and exhaust Bedding Steam autoclaved white pine shavings Photoperiod 12 hours (light) on, 12 hours off; or 12 h light : 12 h dark cycle Relative humidity ~50% Temperature 21-23 °C Health status The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored.