MPD: Wiltshire3: animal documentation

Wiltshire3 animal documentation

Drug study: Neurobiochemical analytes in response to chronic fluoxetine treatment in males of 30 inbred mouse strains (2011)

Wiltshire T, Pletcher MT
With: Benton CS, Miller BH, Skwerer S, Suzuki O, Schultz LE, Cameron MD, Marron JS

Wiltshire3_Animal

Acclimation and testing periods

Vendor Mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, ME)

Acclimation 1 wk

Ages

Mice shipped at 5-6 wks of age; tested 9-11 wks of age

Feed

Pico rodent chow 20; Purina, St Louis, MO, USA, was made available ad libitum

Water

Water was made available ad libitum

Housing
Mice were housed in an SPF barrier colony and in groups of 2-4 in standard high efficiency particulate air-filtered polycarbonate mouse cages.

Bedding

A layer of Bed-o-cob bedding and one cotton nestlet

Humidity 35-65%

Photoperiod

Animals were maintained on a 12-h light: 12-h dark cycle; All behavioral testing was conducted during the light part of the light/dark cycle and occurred between the hours of 1300h and 1600h.

Temperature

22 +/- 1.0°C

Regulatory compliance

All procedures were conducted in strict compliance with the policies on animal welfare of the National Institutes of Health and the University of North Carolina (stated in the "Guide for the Care and Use of Laboratory Animals," Institute of Laboratory Animal Resources, National Research Council, 1996 edition), and approved by the University of North Carolina Animal Care and Use Committee.

Vendor	Mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, ME)
Acclimation	1 wk
Ages	Mice shipped at 5-6 wks of age; tested 9-11 wks of age
Feed	Pico rodent chow 20; Purina, St Louis, MO, USA, was made available ad libitum
Water	Water was made available ad libitum
Housing	Mice were housed in an SPF barrier colony and in groups of 2-4 in standard high efficiency particulate air-filtered polycarbonate mouse cages.
Bedding	A layer of Bed-o-cob bedding and one cotton nestlet
Humidity	35-65%
Photoperiod	Animals were maintained on a 12-h light: 12-h dark cycle; All behavioral testing was conducted during the light part of the light/dark cycle and occurred between the hours of 1300h and 1600h.
Temperature	22 +/- 1.0°C
Regulatory compliance	All procedures were conducted in strict compliance with the policies on animal welfare of the National Institutes of Health and the University of North Carolina (stated in the "Guide for the Care and Use of Laboratory Animals," Institute of Laboratory Animal Resources, National Research Council, 1996 edition), and approved by the University of North Carolina Animal Care and Use Committee.