Shorter8 project protocol

Male reproduction in Diversity Outbred mice: Organ weights and sperm count, motility, and morphology   (2020)

Shorter JR, O'Brien DA, Pardo-Manuel de Villena F
With: Pan W, Odet F, Aylor DL, Kao C-Y, Fu C-P, Greenstein S, Bell TA, Stevans AM, Patel S, Cates SE, Shaw GD, MIller DR, McMillian L

Project protocol - Contents

Workflow and sampling

Step Description Equipment Data collected
1 Mice euthanized and weighed Scales Body weight
2 Reproductive organs harvested and weighed Scales Testis, epididymis with attached vas deferens, and seminal vesicle weights
3 Sperm harvested from right cauda epididymis and counted Hemocytometer Sperm counts
4 Sperm harvested from left cauda epididymis to assess sperm motility Microscope (Computer Assisted Sperm Analysis (CASA)) Sperm motility
5 Sperm from left cauda epididymis prepared to assess morphology Microscopy Sperm morphology


Protocol References

Goodson SG, Zhang Z, Tsuruta JK, Wang W, O'Brien DA. Classification of mouse sperm motility patterns using an automated multiclass support vector machines model. Biol Reprod. 2011 Jun;84(6):1207-15. doi: 10.1095/biolreprod.110.088989. Epub 2011 Feb 23.   PubMed 21349820     FullText