Step Description Equipment Data collected 1 Mice euthanized and weighed Scales Body weight 2 Reproductive organs harvested and weighed Scales Testis, epididymis with attached vas deferens, and seminal vesicle weights 3 Sperm harvested from right cauda epididymis and counted Hemocytometer Sperm counts 4 Sperm harvested from left cauda epididymis to assess sperm motility Microscope (Computer Assisted Sperm Analysis (CASA)) Sperm motility 5 Sperm from left cauda epididymis prepared to assess morphology Microscopy Sperm morphology
Goodson SG, Zhang Z, Tsuruta JK, Wang W, O'Brien DA. Classification of mouse sperm motility patterns using an automated multiclass support vector machines model. Biol Reprod. 2011 Jun;84(6):1207-15. doi: 10.1095/biolreprod.110.088989. Epub 2011 Feb 23.