Shorter8: Male reproduction in Diversity Outbred mice: Organ weights and sperm count, motility, and morphology (2020)

Shorter JR, O'Brien DA, Pardo-Manuel de Villena F   With: Pan W, Odet F, Aylor DL, Kao C-Y, Fu C-P, Greenstein S, Bell TA, Stevans AM, Patel S, Cates SE, Shaw GD, MIller DR, McMillian L

         
Diversity Outbred (DO) mice were assessed in this project. See Shorter4 for the same assessments in DO founder strains (aka Collaborative Cross founder strains).

Procedures conducted:
• body weight 
• organ weights  Male reproductive organ weights. Testes, seminal vesicles, epididymis + vas deferens.
• sperm count  Sperm count. Hemocytometer.
• microscopy  Sperm motility
• histopathology  Male reproductive histopathology. Percent normal, abnormal sperm.

Shorter8 downloads
• Download Shorter8 project data set     animal data, as uploaded
• Download Shorter8 animal data matrix     with factor-related expansion applied as necessary
• Download Shorter8 strain means, SD, N, etc.     one row per strain/sex/measure
Investigators John R Shorter       University of North Carolina,  Chapel Hill, NC
Deborah A O'Brien       University of North Carolina,  Chapel Hill, NC
Fernando Pardo-Manuel de Villena       University of North Carolina,  Chapel Hill, NC
Participants Pan W, Odet F, Aylor DL, Kao C-Y, Fu C-P, Greenstein S, Bell TA, Stevans AM, Patel S, Cates SE, Shaw GD, MIller DR, McMillian L
ContactFernando Pardo-Manuel de Villena     fernando@med.unc.edu     Lab web site
AcknowledgementsFunding provided by NIH HD065024; Eunice Kennedy Shriver National Institute of Child Health and Human Development. We would like to thank Lisa Branstetter, T. Justin Gooch, Stephanie Hansen, Ginger Shaw, Jennifer Shockley and Jason Spence for mouse work.
Project type Phenotype strain survey data set
MPD identifiersShorter8     MPD:658
Data changelog No updates/corrections.       Initial release date: 06/2020.
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Mice: DO population   ♂   age 12-15wks   1 experimental group

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Procedure / Protocol
Treatment
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No. of Strains
Sex
Age
    Shorter8 body weight (none) body weight   [g]  bw   110156     DO population m 12-15wks N=151
    Shorter8 organ weights (none) kidney weight, right   [g]  kidney_wt   110157     DO population m 12-15wks N=147
    Shorter8 organ weights (none) testis weight   [g]  testis   110158     left    right DO population m 12-15wks N=150
    Shorter8 organ weights (none) testis weight mean   [g]  testis_mean   110160     DO population m 12-15wks N=150
    Shorter8 organ weights (none) testis weight as percentage of body weight   [%]  testis_pct_bw   110161     DO population m 12-15wks N=150
    Shorter8 organ weights (none) epididymis plus vas deferens weight   [g]  epidid_vd   110162     left    right DO population m 12-15wks N=150
    Shorter8 organ weights (none) epididymis plus vas deferens weight mean   [g]  epidid_vd_mean   110164     DO population m 12-15wks N=150
    Shorter8 organ weights (none) epididymis plus vas deferens weight as percentage of body weight   [%]  epidid_vd_pct_bw   110165     DO population m 12-15wks N=150
    Shorter8 organ weights (none) seminal vesicles weight total   [g]  semin_vesi_tot   110166     DO population m 12-15wks N=150
    Shorter8 organ weights (none) seminal vesicles weight as percentage of body weight   [%]  semin_vesi_pct_bw   110167     DO population m 12-15wks N=150
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