Shorter3 animal documentation

Litter size and composition in a diallel of Collaborative Cross founder strains   (2018)

Shorter JR, Maurizio PL, Valdar W, Pardo-Manuel de Villena F
With: Bell TA, Shaw GD, Miller DR, Gooch TJ, Spence JS, McMillan L

See also: Shorter3 protocol


Animal documentation

Source Mice originated from a colony maintained by Gary Churchill at The Jackson Laboratory in Bar Harbor, Maine. Mice were bred at the UNC Hillsborough vivarium from 2008-2010 and bred at the UNC Genetic Medicine Building vivarium from 2010-2012.
Ages
3 wks of age (at weaning)
Feed

Purina Prolab RMH3000, provided ad libitum. Mouse chow was supplemented with Fenbendazole two weeks before and two weeks after transportation to the GMB facility to eliminate possible pinworms. Selemectin treatment was dropped onto the coats of mice before transfer to GMB to remove mites.

Water
Provided ad libitum
Temperature
20-24°C
Humidity
40-50%
Housing
Mice were housed in standard 20x30 cm ventilated polysulfone cages
Bedding Bed-O-Cob
Photoperiod 14:10h light:dark cycle (lights on at 0600h)
Testing period 2008-2012
Care and Use
Animal rearing and breeding was conducted in strict compliance with the Guide for the Care and Use of Laboratory Animals (Institute of Laboratory Animals Resources, National Research Council 1996)