Shorter1 animal documentation

Male fertility assessment in 347 pre-CC lines of the Collaborative Cross that are now extinct   (2017)

Shorter JR, O'Brien DA, Pardo-Manuel de Villena F
With: Odet F, Aylor DL, Pan W, Kao CY, Fu CP, Morgan AP, Greenstein S, Bell TA, Stevans AM, Feathers RW, Patel S, Cates SE, Shaw GD, Miller DR, Chesler EJ, McMillian L

Shorter1 protocol

Animal documentation

Source	All funnels were initially bred at Oak Ridge National Laboratory (ORNL) in Oak Ridge TN, and then at the University of North Carolina at Chapel Hill NC since 2009. A single male from each of 347 independent lines (which were declared extinct between 2008 and 2011) was transferred to the laboratory of Fernando Pardo-Manuel de Villena at UNC
Ages	179 - 862 days of age
Feed	Purina Prolab RMH3000, provided ad libitum
Water	Provided ad libitum
Temperature	Not specified
Humidity	Not specified
Housing	Mice were housed in standard 20 x 30 cm ventilated polysulfone cages with laboratory grade Bed-O-Cob bedding
Photoperiod	Not specified
Care and Use	All procedures were performed according to the Guide for the care and Use of Laboratory Animals with prior approval by the Institutional Animal Care and Use Commmittee (Animal Welfare Assurance Number: A-3410-01)