Schughart4 animal documentation

Susceptibility to influenza A (H3N2) viral infection: body weight, viral loads and leukocyte profiles in females of the 8 inbred founder strains of the Collaborative Cross   (2016)

Leist SR, Kollmus H, Schughart K

See also: Schughart4 protocol


Schughart2 Animal Documentation

Acclimation & testing periods

Vendor Breeders obtained from The Jackson Laboratory, Bar Harbor Maine USA; mice were bred at the test facility in Braunschweig for 2-6 generations, depending on the strain.
Ages
7-13 wks (95.5% were 8-12 wks)
Feed
Ssniff #V 1434-300, given ad libitum
Water
Acidified pH 3.0, given ad libitum
Bedding
Aspen wood (Populus tremula) (Tabvei, Estonia, partical size: 5x5x1 mm)
Housing
IVC (Techniplast, blue line)
Cage density
192 cages per room, 3-4 animals per cage
Photoperiod
12h:12h
Temperature
22°C +/- 1°C
Relative humidity
55% +/- 5%
Health status
Specific pathogen free (SPF)
Regulatory compliance
All experiments were approved by an external committee according to German regulations on animal welfare. Protocols were reviewed by an ethics committee (equivalent to IACUC).