MPD: Schughart3: animal documentation

Schughart3 animal documentation

Susceptibility to influenza A (H3N2) viral infection: body weight and survival at three doses in the 8 inbred founder strains of the Collaborative Cross (2013)

Leist SR, Kollmus H, Schughart K

Schughart2 Animal Documentation

Acclimation & testing periods

Vendor Breeders obtained from The Jackson Laboratory, Bar Harbor Maine USA; mice were bred at the test facility in Braunschweig for 2-6 generations, depending on the strain.

Ages
7-13 wks (95.5% were 8-12 wks)

Feed
Ssniff #V 1434-300, given ad libitum

Water
Acidified pH 3.0, given ad libitum

Bedding
Aspen wood (Populus tremula) (Tabvei, Estonia, partical size: 5x5x1 mm)

Housing
IVC (Techniplast, blue line)

Cage density
192 cages per room, 3-4 animals per cage

Photoperiod
12h:12h

Temperature
22°C +/- 1°C

Relative humidity
55% +/- 5%

Health status
Specific pathogen free (SPF)

Regulatory compliance
All experiments were approved by an external committee according to German regulations on animal welfare. Protocols were reviewed by an ethics committee (equivalent to IACUC).

Vendor	Breeders obtained from The Jackson Laboratory, Bar Harbor Maine USA; mice were bred at the test facility in Braunschweig for 2-6 generations, depending on the strain.
Ages	7-13 wks (95.5% were 8-12 wks)
Feed	Ssniff #V 1434-300, given ad libitum
Water	Acidified pH 3.0, given ad libitum
Bedding	Aspen wood (Populus tremula) (Tabvei, Estonia, partical size: 5x5x1 mm)
Housing	IVC (Techniplast, blue line)
Cage density	192 cages per room, 3-4 animals per cage
Photoperiod	12h:12h
Temperature	22°C +/- 1°C
Relative humidity	55% +/- 5%
Health status	Specific pathogen free (SPF)
Regulatory compliance	All experiments were approved by an external committee according to German regulations on animal welfare. Protocols were reviewed by an ethics committee (equivalent to IACUC).