MPD: Schughart1: animal documentation

Schughart1 animal documentation

Susceptibility to influenza A (H1N1) viral infection in females of 9 strains of mice (2009)

Schughart K
With: Srivastava B, Błazejewska P, Hessmann M, Bruder D, Geffers R, Mauel S, Gruber AD

Schughart1 Animal Documentation

Acclimation & testing periods

Vendor C57BL/6J, DBA/2J, FVB/NJ, CBA/J, BALB/cByJ, and (C57BL/6J x DBA/2J)F1 (B6D2F1) were obtained from Janvier, France; SJL/JOrlCrl from Charles River, Germany; DBA/2NHsd from Harlan, Germany; and A/JOlaHsd from Harlan, U.K.

Ages
10-12 wks at the start of the experiment

Feed
Rodent maintenance diet Ssniff® V1534-3 R/M-H, autoclaved, given ad libitum

Water
Acidified with sulfuric acid (pH 3), autoclaved, given ad libitum

Housing
5 mice/cage (530 cm²)

Photoperiod
12hr:12hr day-night cycle (day: 6 a.m. to 6 p.m. CET)

Temperature
22 °C

Relative humidity
55% (exchange rate: 15-17 room volumes/hr)

Health status
Specific pathogen free (SPF)

Regulatory compliance
Mice were maintained under specific pathogen free conditions and according to the German animal welfare law. All experiments were approved by an external committee according to the German regulations on animal welfare.

Vendor	C57BL/6J, DBA/2J, FVB/NJ, CBA/J, BALB/cByJ, and (C57BL/6J x DBA/2J)F1 (B6D2F1) were obtained from Janvier, France; SJL/JOrlCrl from Charles River, Germany; DBA/2NHsd from Harlan, Germany; and A/JOlaHsd from Harlan, U.K.
Ages	10-12 wks at the start of the experiment
Feed	Rodent maintenance diet Ssniff® V1534-3 R/M-H, autoclaved, given ad libitum
Water	Acidified with sulfuric acid (pH 3), autoclaved, given ad libitum
Housing	5 mice/cage (530 cm²)
Photoperiod	12hr:12hr day-night cycle (day: 6 a.m. to 6 p.m. CET)
Temperature	22 °C
Relative humidity	55% (exchange rate: 15-17 room volumes/hr)
Health status	Specific pathogen free (SPF)
Regulatory compliance	Mice were maintained under specific pathogen free conditions and according to the German animal welfare law. All experiments were approved by an external committee according to the German regulations on animal welfare.