Schughart1 animal documentation

Susceptibility to influenza A (H1N1) viral infection in females of 9 strains of mice   (2009)

Schughart K
With: Srivastava B, Błazejewska P, Hessmann M, Bruder D, Geffers R, Mauel S, Gruber AD

See also: Schughart1 protocol


Schughart1 Animal Documentation

Acclimation & testing periods

Vendor C57BL/6J, DBA/2J, FVB/NJ, CBA/J, BALB/cByJ, and (C57BL/6J x DBA/2J)F1 (B6D2F1) were obtained from Janvier, France; SJL/JOrlCrl from Charles River, Germany; DBA/2NHsd from Harlan, Germany; and A/JOlaHsd from Harlan, U.K.
Ages
10-12 wks at the start of the experiment
Feed
Rodent maintenance diet Ssniff® V1534-3 R/M-H, autoclaved, given ad libitum
Water
Acidified with sulfuric acid (pH 3), autoclaved, given ad libitum
Housing
5 mice/cage (530 cm2)
Photoperiod
12hr:12hr day-night cycle (day: 6 a.m. to 6 p.m. CET)
Temperature
22 °C
Relative humidity
55% (exchange rate: 15-17 room volumes/hr)
Health status
Specific pathogen free (SPF)
Regulatory compliance
Mice were maintained under specific pathogen free conditions and according to the German animal welfare law. All experiments were approved by an external committee according to the German regulations on animal welfare.