Animal documentation
Source Breeders were obtained from the System Genetics Core at UNC-Chapel Hill
Provided ad libitum. All diets formulated by Dyets, Inc., Bethleham PA: standard control (#AIN-93G), protein deficient (7.5% casein; #102787), methyl enriched (#518893), vitamin D deficient (#119266)
Mice were grouped housed in standard cages Photoperiod 12:12h light:dark cycle (lights on at 0700h) All procedures and animal care were approved by the UNC Institutional Animal Care an Use Committee and followed the guidelines set forth by the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals