MPD: Data set: Schoenrock1

Schoenrock SA, Oreper D, Farrington J, McMullan RC, Ervin R, Miller DR, Pardo-Manuel de Villena F, Valdar W, Tarantino LM. Perinatal nutrition interacts with genetic background to alter behavior in a parent-of-origin-dependent manner in adult Collaborative Cross mice. Genes Brain Behav. 2018 Sep;17(7):e12438. doi: 10.1111/gbb.12438. Epub 2017 Dec 13. PubMed 29125223

Female mice from selected Collaborative Cross (CC) strains were administered experimental diets: protein-deficient, vitamin D-deficient, methyl donor-enriched or standard (control) diet for 5 wks. These CC females were then crossed to a male from a different CC strain to produce reciprocal F1 hybrid females comprising distinct genetic backgrounds. The diet was administered throughout the gestation period until weaning. The adult F1 females were then tested at 8-9 wks of age in the open field, light/dark, stress-induced hyperthermia, forced swim and restraint stress assays.

Procedures conducted:

• body weight		Body weight at weaning and time of test. Comparison of 4 diets.
• open field test		Locomotor activity, anxiety-related behavior. 10 min test. Comparison of 4 diets.
• light-dark box		Anxiety-related behavior. 10 min test. Comparison of 4 diets.
• body temperature		Temperature before and after stress. Comparison of 4 diets.
• forced swim test		Immobility time. 6 min test. Comparison of 4 diets.
• hormone quantification		Serum corticosterone at baseline and 10 mins after stress. Comparison of 4 diets.

Schoenrock1 downloads
• Download Schoenrock1 project data set animal data, as uploaded
• Download Schoenrock1 animal data matrix with factor-related expansion applied as necessary
• Download Schoenrock1 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Schoenrock1 measures:

MA:0002502 serum
MP:0001259 abnormal body weight
MP:0001362 abnormal anxiety-related response
MP:0001392 abnormal locomotor behavior
MP:0003360 abnormal depression-related behavior
MP:0005345 abnormal circulating corticosterone level
MP:0010165 abnormal response to stress-induced hyperthermia
MP:0020147 abnormal defecation amount
VT:0001259 body mass
VT:0001392 locomotor behavior trait
VT:0005345 blood corticosterone amount
VT:0010462 defecation behavior trait
VT:0010716 anxiety-related behavior trait
VT:0015015 depression-related behavior trait

Investigators	Sarah E Schoenrock University of North Carolina, Chapel Hill, NC Lisa M Tarantino University of North Carolina, Chapel Hill, NC
Participants	Oreper D, Farrington J, McMullan RC, Ervin R, Miller DR, Pardo-Manuel de Villena F, Valdar W
Contact	Lisa M Tarantino lisat@med.unc.edu Lab web site
Acknowledgements	Funding provided by NIH MH100241; Pharmaceutical Research and Manufacturers of America Foundation
Project type	Phenotype strain survey data set
MPD identifiers	Schoenrock1 MPD:612
Data changelog	No updates/corrections. Initial release date: 04/2018.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Schoenrock1:2	Schoenrock1	body weight	various diets	body weight at weaning [g] bw_d21 61202 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	3wks	N=11

Schoenrock1:6	Schoenrock1	body weight	various diets	body weight at start of testing [g] bw_d60 61206 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:10	Schoenrock1	body weight	various diets	body weight change (start of testing minus weaning) [g] bw_chg 61210 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:14	Schoenrock1	open field test	various diets	number of fecal boli, 10 min test [g] OF_boli 61214 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:18	Schoenrock1	open field test	various diets	total distance traveled, 10 min test [cm] OF_dist 61218 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=10

Schoenrock1:22	Schoenrock1	open field test	various diets	number of vertical movements (rearing), 10 min test [n] OF_vert 61222 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=10

Schoenrock1:26	Schoenrock1	open field test	various diets	percentage of time in center of arena, 10 min test [%] OF_center 61226 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=10

Schoenrock1:30	Schoenrock1	light-dark box	various diets	number of fecal boli, 10 min test [n] LD_boli 61230 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:34	Schoenrock1	light-dark box	various diets	number of transitions between light and dark sides, 10 min test [n] LD_trans 61234 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:38	Schoenrock1	light-dark box	various diets	distance traveled in dark side, 10 min test [cm] LD_dist_dk 61238 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:42	Schoenrock1	light-dark box	various diets	distance traveled in light side, 10 min test [cm] LD_dist_lt 61242 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:46	Schoenrock1	light-dark box	various diets	total distance traveled (dark + light), 10 min test [cm] LD_dist_tot 61246 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:50	Schoenrock1	light-dark box	various diets	percentage of time in light side, 10 min test [%] LD_pct_lt 61250 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:54	Schoenrock1	body temperature	various diets	basal body temperature [°C] SIH_temp1 61254 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:58	Schoenrock1	body temperature	various diets	body temperature taken 10 min after stress of taking basal temperature [°C] SIH_temp2 61258 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:62	Schoenrock1	body temperature	various diets	change in temperature (stress minus basal) [°C] SIH_temp_chg 61262 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:66	Schoenrock1	forced swim test	various diets	number of fecal boli, 6 min test [n] FST_boli 61266 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:70	Schoenrock1	forced swim test	various diets	percentage of time spent immobile in the last 4 mins, 6 min test [%] FST_immobile 61270 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:74	Schoenrock1	hormone quantification	various diets	basal serum corticosterone [ng/mL] CORT_basal 61274 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:78	Schoenrock1	hormone quantification	various diets	serum corticosterone after 10 min restraint stress [ng/mL] CORT_stress 61278 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1:82	Schoenrock1	hormone quantification	various diets	change in serum corticosterone (stress minus basal) [ng/mL] CORT_chg 61282 standard diet protein-deficient diet methyl-enriched diet vitamin D-deficient diet	CC RIX	18	f	8-9wks	N=11

Schoenrock1: Perinatal nutrition effects on anxiety- and depression-related behaviors in females of F1 hybrids (RIX) of Collaborative Cross strains of mice (2018)

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