Acclimation & testing periods
VendorMale inbred strains were purchased from the Jackson Laboratory (JAX®, Bar Harbor, ME) at 5-6 wks of age AgesMice were 8-14 wks of age at the start of the study FeedFree access to the water and irradiated NTP-2000 wafer chow (Zeigler Brothers, Inc., East Berlin, PA) WaterWater was given ad libitum HousingIndividually housed in metabolic cages Room temperature20.6-23.9°C ambient temperature Humidity35-65% BeddingNo bedding. Wire-bottom cages. Photoperiod12h:12h light-dark cycle Regulatory complianceCare of mice followed institutional guidelines under a protocol approved by the Institutional Animal Care and Use Committee at the University of North Carolina at Chapel Hill.