Source Mice were obtained from The Jackson Laboratory, Bar Harbor Maine (generation 8 of outcrossing) Ages 11 wks of age at shipping; 13-17 wks at testing Feed
NIH31 5K52 chow, LabDiet/PMI Nutrition, St. Louis MO, provided ad libitum
Water Acidified, provided ad libitum Temperature 23° +/- 1°C Humidity Climate controlled, but not specified Housing Mice were housed in duplex polycarbonate cages with a Shepherd Shack on ventilated racks providing 99.997% HEPA filtered air to each cage. Female mice were group-housed with a cage density of 4-5; male mice were singly housed due to aggressive behavior. Photoperiod 12:12h light:dark cycle (lights on at 0600h) Care and Use All procedures and protocols approved by The Jackson Laboratory Animal Care and Use Committee and conducted in compliance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals.