Recla2: Late phase formalin pain response in a Diversity Outbred (DO) mouse population (2019)

Recla JM, Bubier JA, Gatti DM, Ryan JL, Long KH, Robledo RF, Glidden NC, Hou G, Churchill GA, Maser RS, Zhang ZW, Young EE, Chesler EJ, Bult CJ. Genetic mapping in Diversity Outbred mice identifies a Trpa1 variant influencing late-phase formalin response. Pain. 2019 Aug;160(8):1740-1753. doi: 10.1097/j.pain.0000000000001571.   PubMed 31335644     FullText

Recla2 downloads
• Download Recla2 project data set     animal data, as uploaded
• Download Recla2 animal data matrix     with factor-related expansion applied as necessary
• Download Recla2 strain means, SD, N, etc.     one row per strain/sex/measure
• Recla2 supplementary data
Investigators Jill M Recla       The Jackson Laboratory,  Bar Harbor, ME
Jason A Bubier       The Jackson Laboratory,  Bar Harbor, ME
Daniel M Gatti       The Jackson Laboratory,  Bar Harbor, ME
Jennifer L Ryan       The Jackson Laboratory,  Bar Harbor, ME
Raymond F Robledo       The Jackson Laboratory,  Bar Harbor, ME
Gary A Churchill       The Jackson Laboratory,  Bar Harbor, ME
Elissa J Chesler       The Jackson Laboratory,  Bar Harbor, ME
Carol J Bult       The Jackson Laboratory,  Bar Harbor, ME
Participants Long KH, Garrett AM, Glidden N, Maser RS, Zhang A-W, Burgess RW, Young EE
ContactJill M Recla     Lab web site
AcknowledgementsFunding provided by NIH DA37927, AA18776, CA034196; DOD grant W81XWH-11-1-0762
Project type Phenotype strain survey data set
MPD identifiersRecla2     MPD:627
Data changelog No updates/corrections.       Initial release date: 09/2019.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
Late phase response to formalin injection was measured in Diversity Outbred mice genotyped for over 70,000 SNPs (SNP data can be retrieved from and searching on 'Recla' ). A QTL, Nociq4, was identified which contains the well-known pain gene Trpa1. Functional consequences of sequence variants in Trpa1 were characterized by isoform expression. These qRT-PCR data are available in a supplementary data file.

Procedures conducted:
• nociception assay  Formalin injection.

Mice: DO population   ♀♂   age 13-17wks   1 experimental group