Recla2: Late phase formalin pain response in a Diversity Outbred (DO) mouse population (2019)

Recla JM, Bubier JA, Gatti DM, Ryan JL, Long KH, Robledo RF, Glidden NC, Hou G, Churchill GA, Maser RS, Zhang ZW, Young EE, Chesler EJ, Bult CJ. Genetic mapping in Diversity Outbred mice identifies a Trpa1 variant influencing late-phase formalin response. Pain. 2019 Aug;160(8):1740-1753. doi: 10.1097/j.pain.0000000000001571.   PubMed 31335644     FullText


         
Recla2 downloads
• Download Recla2 project data set     one row per animal, one column per trait (uploaded by Curator)
• Download Recla2 individual animal data     one row per animal, one column per trait (generated from data)
• Download Recla2 strain means, SD, N, etc.     one row per strain/sex/measure (generated from data)
• Recla2 supplementary data
Investigators Jill M Recla       The Jackson Laboratory,  Bar Harbor, ME
Jason A Bubier       The Jackson Laboratory,  Bar Harbor, ME
Daniel M Gatti       The Jackson Laboratory,  Bar Harbor, ME
Jennifer L Ryan       The Jackson Laboratory,  Bar Harbor, ME
Raymond F Robledo       The Jackson Laboratory,  Bar Harbor, ME
Gary A Churchill       The Jackson Laboratory,  Bar Harbor, ME
Elissa J Chesler       The Jackson Laboratory,  Bar Harbor, ME
Carol J Bult       The Jackson Laboratory,  Bar Harbor, ME
Participants Long KH, Garrett AM, Glidden N, Maser RS, Zhang A-W, Burgess RW, Young EE
ContactJill M Recla     jill.recla@jax.org     Lab web site
AcknowledgementsFunding provided by NIH DA37927, AA18776, CA034196; DOD grant W81XWH-11-1-0762
Project type Phenotype strain survey data set
MPD identifiersRecla2     MPD:627
Data changelog No updates/corrections.       Initial release date: 09/2019.
Formatted citation
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Late phase response to formalin injection was measured in Diversity Outbred mice genotyped for over 70,000 SNPs (SNP data can be retrieved from dodb.jax.org and searching on 'Recla' ). A QTL, Nociq4, was identified which contains the well-known pain gene Trpa1. Functional consequences of sequence variants in Trpa1 were characterized by isoform expression. These qRT-PCR data are available in a supplementary data file.

Summary of procedures that were conducted
• nociception assay Formalin injection.
Mice: DO population   ♀♂   age 13-17wks   1 experimental group