Source of Mice: The Jackson Laboratory, Bar Harbor Maine, USA
Age (wks): 4-13 wks at testing
Test Facility: The Jackson Laboratory’s Center for Biometric Analysis (CBA)
Feed: Autoclaved Purina Lab Diet 5K52, provided ad libitum
Water: Reverse osmosis, acidified water given ad libitum
Bedding: Aspen shavings
Cage Type, Distributor, Material, Size: Micro-isolation cages (Thoren Duplex II Mouse Cage #11) on individually ventilated racks (Thoren Caging Systems, Inc.)
Husbandry: Cage sanitation every 2 wks
Cage Density: 5 max
Cage Enrichment: none
Temperature (°C): 20-22
Humidity (%): 30-70%
Light / Dark Cycle: 12:12h, on at 0600h
Health Status and Monitoring: Routine health monitoring confirmed mice were free of viral antibodies, parasites, and opportunistic organisms.
Care and Use Statement: Mice housed at The Jackson Laboratory in accordance with AAALAC international accreditation and the Guide for the Care and Use of Laboratory Animals standards. Experiments approved by the Institutional Care and Use Committee at The Jackson Laboratory.
Subject Identification: Ear notch; subjects were also tail-marked with a non-toxic marker