Peters1 animal documentation

Hematology and clotting time survey in 43 inbred strains of mice   (2001)

Peters LL, Barker JE
With: Svenson K, Von Smith R

See also: Peters1 protocol

Vendor: Mice obtained from The Jackson Laboratory.

Health status report: The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file.

See JAX®Mice Quality Control Programs or the JAX®Mice Catalog for the list of agents monitored.

Mice from JAX production rooms (barrier facilities) were negative for all agents tested, with the following exceptions (number positive mice/total number mice tested):

 Klebsiella oxytocaPasteurella pneumotropica Pneumocystis cariniiTrichomonads
129S1/SvImJ       1/14
AKR/J 4/15      
BALB/cByJ 4/15      
BALB/cJ 2/15      
BTBR T+ tf/J     2/27  
C57BL/6J 4/15     1/14
C57BL/10J 2/15      
CBA/J 2/15      
MOLF/EiJ* 1/18 11/18   1/18
NOD/ShiLtJ 2/15      
SJL/Bm*   9/12   2/12
WSB/EiJ* 1/18 11/18   1/18
* Maintained in research facility rooms (non-SPF) at The Jackson Laboratory.

Environment before shipping:

Feed: Most strains maintained on a 6% fat diet (Purina 5K52 except the following strains maintained on a 4% fat diet (Purina 5K54): C57BR/cdJ, DBA/1J, NZW/LacJ, and MOLF/EiJ. RIIIS/J alternates weekly between 4% and 6% fat diets, and WSB/EiJ alternates weekly between 4% and 11% (Purina 5K20 fat formulations.

Water: Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/ml) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA).

Housing: Thoren cages; Thoren Maxi Miser #3 cages used for some strains as part of a PIV (pressurized individually ventilated) system.

Bedding: Steam autoclaved white pine shavings.

Photoperiod: 14 hours on (generally covering the hours of 4:30 AM to 6:30 PM) 10 hours off.

Temperature: 18 - 24 °C

Relative humidity: ~50%.

Acclimation period

Duration: 10-14 days (barrier research facility room G3A at The Jackson Laboratory).

Water: Adjusted with hydrochloric acid to pH 3.0; supplemented with vitamin K.

Feed: Sterilized LabDiet 5K52.

Housing: same as before shipping (above).

Bedding: same as before shipping (above).

Photoperiod: 12 hours on (6 am to 6 pm), 12 hours off.

Temperature: 24 - 25 °C

Relative humidity: 47-49%

Health status report: Mice appeared healthy during acclimation period.

Testing period

    Hematology   08-Jan-2001 to 12-Sep-2002
    Coagulation   02-Mar-2001 to 19-Mar-2002