Standard chow diet
(Purina LabDiet, 5010, St. Louis, MO, USA)
High-fat High-sucrose (HFHS) diet
(Research Diets, D12331, New Brunswick, NJ, USA)
Steps
All mice are fed the chow diet until 4-5 weeks of age.
At 4-5 wks of age, all mice are fed the HFHS diet until the end of the study at 18-19 weeks of age.
Procedure 2: Body weight and size determination
Equipment, software, and supplies
Balance scale
Measuring ruler
Reagents and solutions
Avertin, 0.8 mg/g of 2,2,2,-tribromoethanol in tertiary-amyl alcohol
(Fisher Scientific, Pittsburgh, PA, USA)
Steps
Mice are weighed on a weekly basis 4-5 wks of age up to 18-19 wks of age.
Mice at 18-19 wks of age are moved to clean cages, fasted overnight, weighed, and then anesthetized via intraperitoneal injection with Avertin. Nose to anus length is measured to calculate BMI.