Molenhuis1 project protocol

Social interaction and locomotor activity, digging, and grooming in males of 52 Collaborative Cross strains of mice   (2018)

Molenhuis RT, Iraqi FA, Mott RF, Kas MJ
With: Bruining H, Brandt MJV, van Soldt PE, Abu-Toamih Atamni HJ, Burbach JPH

Project protocol - Contents

Workflow and sampling

Step Description Equipment Data collected
1 Social interaction and discrimination capacity determined Monitoring system Time exploring unfamiliar conspecific mouse, preference for novel versus familiar conspecific mouse
2 Locomotor activity and exploratory behaviors assessed with four novel objects Monitoring system Distance traveled, time digging, time grooming
3 Mice weighed and euthanized; brains harvested and weighed Balance Body weight, brain weight


Primary References

Molenhuis RT, Bruining H, Brandt MJV, van Soldt PE, Abu-Toamih Atamni HJ, Burbach JPH, Iraqi FA, Mott RF, Kas MJH. Modeling the quantitative nature of neurodevelopmental disorders using Collaborative Cross mice. Mol Autism. 2018 Dec 13;9:63. doi: 10.1186/s13229-018-0252-2. eCollection 2018.   PubMed 30559955     FullText

Protocol References

Molenhuis RT, de Visser L, Bruining H, Kas MJ. Enhancing the value of psychiatric mouse models; differential expression of developmental behavioral and cognitive profiles in four inbred strains of mice. Eur Neuropsychopharmacol. 2014 Jun;24(6):945-54. doi: 10.1016/j.euroneuro.2014.01.013. Epub 2014 Jan 18.   PubMed 24491952