Project protocol - Contents
- Workflow and sampling
- Equipment and supplies
- Reagents and solutions
- Procedure: von Frey testing
- Procedure: Spared nerve injury (SNI) and hypersensitivity testing
- Definitions and calculations
Step Procedure performed Data collected 1 von Frey testing baseline 2 Spared nerve injury procedure - 3 von Frey testing on post-operative days (1, 4, 7, 14, 21, 28) % allodynia
- Surgical equipment
- von Frey testing cubicles (see details below)
- Nylon monofilaments (Stoelting Touch Test Sensory Evaluator Kit)
Mice habituated to the vivarium for at least one week before testing,
- Mice are placed individually in transparent Plexiglas cubicles (5 cm wide x 8.5 cm long x 6 cm high, where each cubical is separated from another by an opaque divider) placed on a perforated metal floor (with 5 mm diameter holes placed 7 mm apart).
- Mice are habituated at least 2 h before testing.
- Nylon monofilaments (corresponding to 0.015 to 1.3 g bending force; calibrated weekly) are firmly applied to the plantar surface of the hindpaw (alternating the side of the body being tested), until they bowed for 5 s.
- Mice are tested only when alert or resting, not while grooming.
- Three separate threshold determinations are made and averaged.
- Mice are anesthetized with isoflurane and oxygen.
- Mice receive unilateral SNI according to published procedures (Shields et al., 2003).
- Von Frey fibers are applied to the lateral aspect of the plantar hindpaw (as detailed above) on post-operative days 1, 4, 7, 14, 21, and 28.
- baseline hypersensitivity
- 50% allodynia
Shields SD, Eckert WA 3rd, Basbaum AI. Spared nerve injury model of neuropathic pain in the mouse: a behavioral and anatomic analysis. J Pain. 2003 Oct;4(8):465-70.