McMullan7 animal documentation

Energy balance traits after forced exercise in 4 Collaborative Cross strains of mice   (2018)

McMullan RC, Pardo-Manuel de Villena F
With: Ferris MT, Bell TA, Menachery VD, Baric RS, Hua K, Pomp D, Smith-Ryan AE

McMullan7 protocol

Animal documentation

Source	All mice were purchased from the Systems Genetics Core Facility (http://csbio.unc.edu/CCstatus/index.py) and housed in the Division of Comparative Medicine facilities at the University of North Carolina at Chapel Hill. Note that strain names are purposely not given the 'J' designation as they were obtained from UNC.
Ages	8-10 wks of age at start of experiment
Feed	Envigo 2920 irradiated chow, provided ad libitum
Water	Provided ad libitum
Temperature	23° +/- 1°C
Humidity	Humidity controlled, but not specified
Housing	Mice were group housed
Photoperiod	12:12h light:dark cycle (lights on at 0700h)
Time period	Mice were born March to August 2016 and February to March 2017. The experiment was performed in 5 batches. Every strain, sex, exercise program combination was represented at least once in each batch for batches 1-4. In order to increase biological replicates, an additional batch was added.
Care and Use	All procedures performed within this experiment were approved by the University of North Carolina – Chapel Hill Institutional Animal Care and Use Committee