Mathes1 project protocol

Energy balance traits in 8 inbred founder strains and 170+ emerging lines (pre-CC) of the Collaborative Cross   (2011)

Mathes WF
With: Aylor DL, Miller DR, Churchill GA, Chesler EJ, de Villena FP, Threadgill DW, Pomp D


Project protocol — Contents
Workflow and sampling
Equipment and supplies
Reagents and solutions

Workflow and sampling


Procedure performed
Data collected
Mice housed in cages without access to running wheel (sedentary)
acclimation period
Average daily food intake measured (Fig. 1)
daily food intake before exercise
Mice weighed and scanned for body fat
scale, MRI
body weight, percent body fat
Mice subjected to indirect calorimetry for 24 h
calorimeter chamber
respiratory exchange ratio (RER)
Mice returned to cages with attached running wheels and allowed monitored access for 12 days; average daily food intake measured
running wheel with activity monitor system
wheel running activity and daily food intake during exercise
Mice weighed and scanned for body fat; prevented access to running wheel
scale, MRI
post-exercise body weight, percent body fat (data not submitted)
Mice subjected to indirect calorimetry for 24 h
calorimeter chamber
post-exercise RER (data not submitted
Mice sacrificed
*Adapted from Table 1. Mathes et. al., 2012

Figure 1. Experimental timeline (not drawn to scale).

Equipment and supplies

  • Spill-resistant feeders (OYC Rodent Café; OYC International, Andover, MA)
  • Standard laboratory cages with attached running wheels (Mouse Activity Wheel Chamber; Lafayette Industries, Lafayette, IN)
  • Balance scale
  • Calorimetric chambers (TSE calosys; TSE Systems, Germany)
  • Automated activity wheel monitoring program (AWM; Lafayette Industries, Lafayette, IN)
  • Magnetic resonance imaging (MRI) (EchoMRI, Houston, TX)
  • Cleaning supplies

Reagents and solutions

  • 95% Ethanol
  • Disinfectant or cleaning solution

Acclimation to test conditions

Mice are acclimated to the mouse facility for at least 8 days following their arrival.


Exercise and metabolism data collected as part of a highthroughput phenotyping screen.
Body weight (BW) is measured before and at the end of wheel running activity (Fig. 1).

. Likewise, body composition is evaluated for fat and lean mass percentages before the start of wheel running and again afterwards using magnetic resonance imaging (MRI).
For easier spill detection and collection, standard laboratory chow is ground, dyed blue, and presented in spill-resistant feeders. Any spillage is collected and weighed.
Daily average food intake is calculated for 3 days prior to wheel running activity by dividing the total amount of food consumed over the 3-day period.
The day before mice are given access to running wheels they are placed into calorimetric chambers and O2 consumption, CO2 production and respiratory exchange ratios (RER) are measured for 24 h.
Wheel running is recorded continuously over a 12-day period using an automated activity wheel monitoring system.
During the 12 days of wheel running, food intake is measured every other day, and daily averages are calculated.
Change in BW and percent body fat in response to exercise are calculated by subtracting the pre-exercise value from the post-exercise value. Negative values indicate a loss of BW or fat in response to wheel running.
Food intakes are corrected for body weight by dividing average intakes by the average BW for the measurement period.
Distance and speed data are directly extracted for day 1, and running distances and speeds are averaged for days 5 and 6 and days 11 and 12 of wheel running activities.

Definitions and calculations

Collaborative Cross (CC): is an emerging mouse recombinant inbred (RI) panel that was designed to extend the available mouse genetic reference populations (Philip et al. 2012) by combining the genomes of eight genetically and phenotypically diverse strains through randomized breeding design that systematically outcrosses eight founder strains, followed by inbreeding to obtain new recombinant inbred strains.

Founder strains: five common inbred (A/J, C57BL/6J, 129S1/SvImJ, NOD/ShiLtJ, NZO/HlLtJ) and three wild-derived inbred (CAST/EiJ, PWK/PhJ, WSB/EiJ) mice

Respiratory exchange ratio (RER): ratio between the amount of O2 inhaled and CO2 exhaled in one breath, an indirect measurement of energy expenditure.

Data collected by investigator

  • caloric intake
  • respiratory exchange ratio
  • distance traveled
  • speed
  • performance difference