Acclimation and testing periods
Supplier Inbred mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, Maine). Collaborative Cross mice bred and obtained from Oak Ridge National Laboratory (Oak Ridge, TN 37831). Ages Male mice 10-12 wk of age Feed Mice given ad libitum access to food and ground standard laboratory chow, dyed blue for easier spill detection and collection (LabDiet 5053, blue; TestDiet, Richmond,IN), and presented in spill-resistant feeders (OYC Rodent Café; OYC International, Andover, MA) Water Mice given ad libitum access to water Housing Mice housed individually in standard laboratory cages with attached running wheels (1.1 m diameter; Lafayette Industries, Lafayette, IN). Access to the running wheels was blocked for the first 8 days after the mice arrived in the vivarium. Photoperiod 12 h (light) ON, 12 h (without light) OFF in a light-dark cycle with lights ON at 0600 h Relative humidity Controlled Temperature Maintained 21±1°C Regulatory compliance All procedures performed within this series of experiments were approved by the University of North Carolina - Chapel Hill Institutional Animal Care and Use Committee.