Lake3 animal documentation

Behavioral and neuromuscular phenotypes, C57BL/6J-Chr#A/J/NaJ mouse chromosome substitution panel   (2005)

Lake J, Donahue L, Davisson MT

See also: Lake3 protocol


Lake3 Animal Documentation

  Acclimation & testing periods

Ages
8-9 wks
Feed
6% fat diet (Purina 5K52)
Water
Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/ml) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA).
Housing
Thoren cages; Thoren Maxi Miser #3 cages used for some strains as part of a PIV (pressurized individually ventilated) system.
Bedding
Steam autoclaved white pine shavings
Photoperiod
12 hr on 12 hrs off – 5:00 AM On – 5:00 PM Off
Temperature
18 - 24°C
Relative humidity
~50%
Health status
The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored.