Step Description Equipment Data collected 1 Acclimation in test room (30-45 min) - - 2 Open field test Open field arena Locomotor activity
Equipment, software, and supplies
- Open field chamber, 52 x 52 x 23 cm; floor is PVC plastic and walls are gray PVC plastic; 600 lux
- Actimetrics LimeLight software
Reagents and solutions
- Dilute Quatricide solution, for cleaning chamber (Pharmacal Research Labs)
- Mice are acclimated to test room for 30-45 min prior to testing.
- Activity is measured for 10 min and recorded; mice are allowed to explore for a total of 55 min.
Geuther BQ, Deats SP, Fox KJ, Murray SA, Braun RE, White JK, Chesler EJ, Lutz CM, Kumar V. Robust mouse tracking in complex environments using neural networks. Commun Biol. 2019 Mar 29;2:124. doi: 10.1038/s42003-019-0362-eCollection 2019.