Jaxpheno16: Multi-system assessment of B6.BKS(D)-Leprdb/J mice (2018)

Jackson Laboratory

   
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Institutional authorship Jackson Laboratory
ContactAndrew J Schile     andrew.schile@jax.org
AffiliationJAX Phenotyping Center
Project type One-strain study
MPD identifiersJaxpheno16     MPD:611
Data changelog No updates/corrections.       Initial release date: 02/2018.
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Purpose: To characterize B6.BKS(D)-Leprdb/J (db/db) mice between the ages of 4 and 20 weeks. Heterozygous mice (db/+) were tested as controls. Mice were screened for obesity, non-fasting glucose levels, body composition, serum lipids, HbA1c (NGSP%), and splenocyte populations. Per-animal data are available in the download file.

Methods

Diet administration: All mice were fed LabDiet 5K52 formulation (6% fat by weight) from the ages of 4 to 20 weeks.

Body weight: At least 40 mice per genotype and sex were measured on the same day each week.

Non-fasted blood glucose: Glucose levels were determined weekly on at least 40 non-fasted mice per genotype and sex. Submandibular blood glucose measurements were obtained using a OneTouch Ultra 2 or UltraMini hand-held glucometer which was validated with a control solution on each day of use.

Body composition: Mice were analyzed using a Lunar PIXImus DEXA scanner. Calculations of body composition excluded the head.

Clinical chemistry: All values were measured from serum collected from submandibular blood except HbA1c, which was measured from submandibular whole blood. Ten to twenty non-fasted mice were tested per genotype and sex. Results were obtained using a Beckman Coulter AU680 chemistry analyzer.

Spleen flow cytometry: All cell populations were calculated as percentages of the total viable splenocyte population, except for regulatory T cells which were calculated as the percentage of viable CD4+ cells. Parameters were measured using a BD Biosciences LSR II flow cytometer using a protocol described here.

Available download (per animal data):  

Jaxpheno16.xlsx