Jaxpheno12: Type 1 diabetes incidence and other phenotypes in NOD/ShiLtJ mice on a 6% fat diet (2018)

Jackson Laboratory   Andrew J Schile

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Institutional authorship Jackson Laboratory
Investigators Andrew J Schile       The Jackson Laboratory -- West,  Sacramento, CA
ContactAndrew J Schile     andrew.schile@jax.org
Affiliated CenterJAX Mice Clinical and Research Services (JMCRS)
Project type One-strain study
MPD identifiersJaxpheno12     MPD:607
Data changelog No updates/corrections.       Initial release date: 02/2018.
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Purpose: To assess effects relevant to type 1 diabetes in a longitudinal study on NOD/ShiLtJ male and female mice fed a 6% fat diet (LabDiet® 5K52) between the ages of 3 and 30 weeks. Per-animal data are available in the download file.


One hundred twenty female and eighty male mice were obtained at weaning (birthdate +/- 3 days) from colonies in the Bar Harbor (standard barrier) and Sacramento (maximum barrier) facilities of The Jackson Laboratory.  Mice were fed a 6% fat diet (LabDiet® 5K52 formulation), ad libitum.  Mice were weighed weekly until 30 weeks of age, on the same day each week. Diabetes onset was also monitored between the ages of 3 and 30 weeks.  Diabetes was defined as a non-fasting blood glucose level exceeding 250 mg/dL.  Blood was sampled in the morning from the submandibular route and analyzed using a OneTouch Ultra2 or UltraMini handheld glucometer that was validated using a control solution on each measurement day.  Data were combined from two independent studies that each included 60 females and 40 males.  Median onset was 18 weeks for females and 30 weeks for males (Figure 1).  The Kaplan-Meier curves did not differ significantly between the individual studies (conducted in Standard and Maximum barrier rooms).  Mice were removed from the study when they became diabetic, developed adverse clinical signs, or were used for phenotyping projects at the ages of 8, 16 and 30 weeks.  Mice used for phenotyping were weighed and body composition was analyzed using a Lunar PIXImus DEXA scanner. Calculations of body composition excluded the head. Cardiac blood was collected under isoflurane anesthesia and divided for hematology (using a Siemens Advia 2120 or Drew Scientific Hemavet 950 FS analyzer) or serum biochemistry (Beckman AU680 analyzer). Single-cell suspensions were also analyzed by flow cytometry following a protocol found here.

Available download (per animal data):   Body weights, blood glucose readings, DXA body composition, spleen flow cytometry, serum clinical chemistry, and hematology.


Figure 1. Diabetes incidence for NOD/ShiLtJ males and females.