JaxCC1 project protocol for procedure: urinalysis

Multisystem phenotyping of 18 Collaborative Cross strains   (2018)

The Jackson Laboratory

See also: JaxCC1 animal documentation         All protocol docs for JaxCC1


See JAX Pipeline on the IMPC website for order of tasks for all mice tested

Project protocol: Urinalysis



Introduction

Urinalysis is performed to assess renal function.



Study design

  • Paradigm: Baseline study
  • Panel: Collaborative Cross
  • Number of strains: 14
  • Sex: Both
  • Age: 12-13 wks
  • Sample size: Five mice per sex from at least two litters
  • Number of cohorts: 1



    Workflow

    Step Description Equipment Data collected
    1 Urine collection (non-fasting), always in the morning - -

    2

    Urinalysis Clinical chemistry analyzer Urine glucose, magnesium level, creatinine, microalbumin, albumin-creatinine ratio


    Equipment, software and supplies


    Reagents and solutions

    Test Au# Test Kit # Calibrator Kit # QC Kit # Frequency of Assay
    Creatinine 12.CRE Beckman OSR6178 Beckman DR0091 BioRad 395X (2 levels) Calibrator and Controls assayed for every day of a test run.
    Microalbumin 27.MALB Beckman OSR6167 Beckman DR3024 BioRad 395X (2 levels) Calibrator and Controls assayed for every day of a test run.
    Magnesium 20.MG Beckman OSR6789 Beckman DR0090 BioRad 395X (2 levels) Calibrator and Controls assayed for every day of a test run.
    Glucose 92.GLUX Beckman OSR6121 Beckman DR0090 BioRad 395X (2 levels) Calibrator and Controls assayed for every day of a test run.

     

    Procedure

    1. Set up the clinical chemistry analyzer and perform QC analysis of the control reagents in accordance with equipment guidelines.
    2. At least 70 µL urine is collected into a microcentrifuge tube (collection is in the morning).
    3. Samples are kept on ice until analysis.
    4. Samples are analyzed on day of collection, undiluted.


    Measurements

    LIMS field name MPD variable name Description Units Series Primary measurement Series order MPD computed Calculation Method Data type
    microAlbumin (calculated) MA microalbumin (urine MA) mg/dL           urinalysis Float

    Creatinine

    CREA creatinine (urine CREA) mg/dL           urinalysis Float
    Albumin to Creatinine ratio ACR albumin-creatinine ratio (urine ACR) mg/dL           urinalysis Float
    Magnesium urine_Mg magnesium level (urine Mg) mg/dL           urinalysis Float
    Glucose urine_GLU glucose (urine GLU) mg/dL           urinalysis Float


    Experimental and technical parameters

    Equipment name Clinical chemistry analyzer
    Equipment manufacturer Beckman Coulter
    Equipment model AU 680
    Fasting None
    Anesthesia None
    Method of urine collection Gentle restraint
    Sample status Fresh (on ice)
    Urine dilution No