JaxCC1 project protocol for procedure: holeboard

Multisystem phenotyping of 18 Collaborative Cross strains   (2018)

The Jackson Laboratory


See JAX Pipeline on the IMPC website for order of tasks for all mice tested

Project protocol: Hole board test


The hole board test monitors the activity of mice over a defined period of time in order to assess exploratory and attentional phenotypes.

Study design

  • Paradigm: Baseline study
  • Panel: Collaborative Cross
  • Number of strains: 7
  • Sex: Both
  • Age: 8-9 wks
  • Sample size: Seven mice per sex from at least two litters
  • Number of cohorts: 1


    Step Description Equipment Data collected
    1 Mice are acclimated to test room for at least 3 days - -


    Each mouse is placed in the center of the arena Hole board -
    3 Behavior is recorded for 20 min Hole board Number of hole pokes

    Equipment, software and supplies

    Reagents and solutions

    • 1% Virkon S solution



    1. Mice are allowed to acclimate to the testing room for a minimum of 3 days.
    2. Photobeam sensor check is performed at the start of each test day or in the event of drift in control parameters using the built-in diagnostic function.
    3. Each mouse is placed in the center of the arena facing to the left of the experimenter and released.
    4. A 20-min recording period is started after all mice have been placed within an arena.
    5. At the end of the 20-min trial, mice are removed from the arenas and returned to their home cages.
    6. Chambers are cleaned with 1% Virkon S solution between mice to decontaminate and remove scent cues from previously tested mice.
    7. Analysis of the recording is done to measure the activity of each mouse.

    Data collection: The Accuscan Versamax system in configured to report parameters for each mouse. Versamax files are converted into text data files using Versadat. The program is used to output an ACT file (contains general movement information) and a HPS file (contains nose poke sequence information).

    Data QC: Verify that total time of each run is 20 min.



    LIMS field name MPD variable name Description Units Series Primary measurement Series order MPD computed Calculation Method Data type
    Total holepokes hole_pokes number of hole pokes, 20 min test n           hole board test Integer

    Experimental and technical parameters

    Light level in light zone 100-200 lux
    Equipment name Nose Poke Holeboard (16 holes; 4 x 4 arrangement)
    Equipment manufacturer Accuscan
    Equipment model Accuscan Versamax
    Start time 0700 - 1700 EST
    Disinfectant 1% Virkon Solution