See JAX Pipeline on the IMPC website for order of tasks for all mice tested
Project protocol: Hole board test
The hole board test monitors the activity of mice over a defined period of time in order to assess exploratory and attentional phenotypes.
Paradigm: Baseline study
Panel: Collaborative Cross
Number of strains: 7
Age: 8-9 wks
Sample size: Seven mice per sex from at least two litters
Number of cohorts: 1
||Mice are acclimated to test room for at least 3 days
|Each mouse is placed in the center of the arena
||Behavior is recorded for 20 min
||Number of hole pokes
Equipment, software and supplies
Reagents and solutions
- Mice are allowed to acclimate to the testing room for a minimum of 3 days.
- Photobeam sensor check is performed at the start of each test day or in the event of drift in control parameters using the built-in diagnostic function.
- Each mouse is placed in the center of the arena facing to the left of the experimenter and released.
- A 20-min recording period is started after all mice have been placed within an arena.
- At the end of the 20-min trial, mice are removed from the arenas and returned to their home cages.
- Chambers are cleaned with 1% Virkon S solution between mice to decontaminate and remove scent cues from previously tested mice.
- Analysis of the recording is done to measure the activity of each mouse.
Data collection: The Accuscan Versamax system in configured to report parameters for each mouse. Versamax files are converted into text data files using Versadat. The program is used to output an ACT file (contains general movement information) and a HPS file (contains nose poke sequence information).
Data QC: Verify that total time of each run is 20 min.
|LIMS field name
||MPD variable name
||number of hole pokes, 20 min test
||hole board test
Experimental and technical parameters
|Light level in light zone
||Nose Poke Holeboard (16 holes; 4 x 4 arrangement)
||0700 - 1700 EST
||1% Virkon Solution