JaxCC1 project protocol for procedure: ABR test

Multisystem phenotyping of 18 Collaborative Cross strains   (2018)

The Jackson Laboratory

See also: JaxCC1 animal documentation         All protocol docs for JaxCC1


See JAX Pipeline on the IMPC website for order of tasks for all mice tested

Project protocol: Auditory brainstem response



Introduction

Auditory brainstem response test determines hearing sensitivity and other physiological parameters using evoked potential recordings in anesthetized mice.

Study design

  • Paradigm: Baseline study
  • Panel: Collaborative Cross
  • Number of strains: 18
  • Sex: Both
  • Age: 15-17 wks
  • Sample size: Three mice per sex
  • Number of cohorts: 1



    Workflow

    Step Description Equipment Data collected
    1 Mice are anesthetized and weighed - -

    2

    Each mouse is prepped for ABR Auditory brainstem response system -
    3 ABRs are recorded Auditory brainstem response system Auditory brainstem response at 6, 12, 18, 24, 30 kHz


    Equipment, software and supplies

    • Auditory brainstem response system [See Experimental and technical parameters]
      • Audio signal generators, amplifiers and loudspeakers
      • Calibration equipment (microphone, etc)
      • EEG Needle electrodes
      • Biological amplifier and headstage
      • Sound attenuating chamber
      • Heating blanket
    • Data acquisition hardware
    • Software to control signal presentation and data acquisition
    • Data Analysis software/database application


    Procedure

    1. Mice are anesthetized i.p. with ketamine-xylazine.
    2. Each mouse is placed on a heating blanket in a sound chamber and sub dermal needle electrodes are inserted (active electrode on vertex, reference electrode overlying left bulla, ground electrode overlying right bulla).
    3. Each mouse is placed unrestrained in a prone position, nose forward, at the calibrated distance from the leading edge of the speaker to the mouse's interaural axis, on a thermostatically controlled blanket, inside a sound attenuating booth.
    4. ABRs are recorded for the following frequencies: 6, 12, 18, 24, 30 kHz.

     

    Data QC: Once or twice annually, the calibration of the microphone used for sound delivery should be checked using a Bruel and Kjaer PULSE system with the Type 4231 calibrator or other equivalent calibration instrumentation suitable for use with high frequencies.

     


    Measurements

    LIMS field name MPD variable name Description Units Series Primary measurement Series order MPD computed Calculation Method Data type
    Body weight bw body weight g           body weight Float
    6kHz-evoked ABR threshold pip_6 auditory brainstem response (ABR) threshold, sound pressure level, 6 kHz pip dB Yes pip_6 1     ABR test Float

    12kHz evoked ABR threshold

    pip_12 auditory brainstem response (ABR) threshold, sound pressure level, 12 kHz pip dB Yes pip_6 2     ABR test Float
    18kHz evoked ABR threshold pip_18 auditory brainstem response (ABR) threshold, sound pressure level, 18 kHz pip dB Yes pip_6 3     ABR test Float
    24kHz evoked ABR threshold pip_24 auditory brainstem response (ABR) threshold, sound pressure level, 24 kHz pip dB Yes pip_6 4     ABR test Float
    30kHz evoked ABR threshold pip_30 auditory brainstem response (ABR) threshold, sound pressure level, 30 kHz pip dB Yes pip_6 5     ABR test Float


    Experimental and technical parameters

    Equipment name Auditory brainstem response system
    Equipment manufacturer Intelligent Hearing Systems
    Equipment model Smart EP
    Software Smart EP
    Recording environment Sound proof booth
    Stimulus level 5
    Anesthesia Ketamine-Xylazine