Acclimation & testing periods
Vendor Mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, ME) as part of a collaboration with the Mouse Phenome Project 8-12 wks at the start of the experiment 6% fat diet (Purina 5K52) Acidified water (pH 2.8-3.2) was made available ad libitum Thoren cages; Thoren Maxi Miser #3 cages used for some strains as part of a PIV (pressurized individually ventilated) system. Steam autoclaved white pine shavings 12 hr on 12 hrs off – 5:00 AM On – 5:00 PM Off 18 - 24°C ~50% The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored. All housing and experimental procedures were conducted under the guidelines of the Canadian Council of Animal Care and were approved by the Biotechnology Research Institute (BRI) Animal Care Committee (Protocol number: 08-SEP-I-017) and the Animal Care Committee of McGill University (Protocol number: 5618).