Gershenfeld1 animal documentation

Drug study: Imipramine effects in 12 inbred strains of mice measured by tail suspension, light-dark transition, and open field tests   (2003)

Gershenfeld HK
With: Liu X

See also: Gershenfeld1 protocol


Acclimation & testing periods

Vendor
Mice were obtained from The Jackson Laboratory JAX® (Bar Harbor, ME) at 4-7wks of age. Male inbred NMRI and 129S6/SvEvTac mice were obtained from B & K Universal (Grimston, Hull, East Yorkshire, UK) and Taconic (Germantown, NY). They were identified by ear notching at 4–7wks of age, and allowed to adapt to their new housing conditions for at least 1 week before testing.
Ages at testing
5–10 wks of age
Feed
Available ad libitum
Water
Available ad libitum
Housing
All mice of the same strain and sex are housed in groups of five
Photoperiod
The animals were maintained under 12h-light/12h-dark cycle with lights on at 6:00 am. Mice were tested individually during the light cycles between 11:00 am and 5:00 pm at 8–9wks of age.
Acclimation
All animals were brought to the testing room at least 1 hr before the commencement of each behavioral test, and remained in the same room throughout the test
Temperature
21.3 - 23.4°C
Regulatory compliance
All experiments followed the National Institutes of Health Guide for Care and Use of Laboratory Animals and were approved by the local institutional review committee